Effects of L-741,741, a selective dopamine receptor antagonist, on anxiety tested in the elevated plus-maze in mice.

Dopamine systems are involved in modulation of anxiety. Although the action of D1/5, D2 and D3 receptor antagonists on anxiety has been documented in different animal models, little is known about the influence of D4 dopamine antagonists. The aim of this study was to examine the effects of L-741,741 (0.75, 1.5 and 3 mg/kg i.p.), a selective D4 dopamine receptor antagonist, on anxiety tested in the elevated plus-maze. Forty-eight albino adult male mice of the OF.1 strain were used. Animals were randomly allocated to one control group receiving physiological saline and dymethyl-sulphoxide (DMSO) (10%) and three experimental groups receiving L-741,741 injections. Tests were performed 30 min after injections for a duration of 5 min. A number of classical parameters were collected: i) open arm duration; ii) closed arm duration; iii) central platform duration; iv) open arm frequency; v) closed arm frequency; vi) central platform frequency; and vii) number of rearings. L-741,741 did not produce any significant behavioral changes under present test conditions, suggesting that the dopamine D4 receptor might not be involved in the modulation of anxiety-related behaviors in the plus-maze.