Voltage-clamp-controlled current-clamp recordings from neurons: an electrophysiological technique enabling the detection of fast potential changes at preset holding potentials.

Investigations of the properties of fast, transient potential changes (e.g. receptor potentials or synaptic potentials) in excitable cells by means of current-clamp recording techniques require the exact adjustment and control of membrane potentials. Usually, the desired membrane potential values are set by current injection via the recording electrode and are controlled manually by regulating the current strength necessary to maintain a constant potential. However, this technique is associated with a number of disadvantages. A single-electrode current- and voltage-clamp amplifier was therefore modified to compensate for slow membrane potential changes without affecting faster voltage responses. Basically, low-pass filters with selectable time constants were incorporated into the voltage-clamp feedback circuit to control the amplifier's response speed. In addition, the amplifier's electronic circuits were altered to enable current pulse injection into the cells. Thus, while recording at preset and controlled membrane potentials, it was possible to monitor the cell's input resistance or current/voltage relationship. This new recording technique has been designated "voltage-clamp-controlled current clamp" (VCcCC) and its performance was tested by intracellular recordings from neocortical and neostriatal neurons in vitro using either conventional microelectrodes or patch-clamp electrodes.