| Literature DB >> 12689717 |
W Van Dessel1, L Van Mellaert, N Geukens, J Anné.
Abstract
Streptomycetes are attractive microorganisms because of their high secretion capacity and for the production of secondary metabolites. We report the improvement of a PCR-based method for screening of solid media-grown Streptomyces transformants. By resuspending mycelium into 2.5-10% dimethyl sulfoxide (DMSO), extensive manipulation prior to PCR could be avoided, thus significantly reducing screening time. Results proved to be both reliable and specific.Entities:
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Year: 2003 PMID： 12689717 DOI： 10.1016/s0167-7012(02)00235-x
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363