OBJECTIVE: To quantitatively measure male DNA in blood from women with systemic sclerosis (SSc) and from controls and to evaluate in vitro the modulation of the microchimeric cell population size following immunologic stimuli that were expected to trigger antigen-specific T cells. METHODS: A real-time polymerase chain reaction for a Y chromosome sequence was used to measure male DNA in blood from women with SSc and from controls who gave birth to sons. The in vitro change in the microchimeric cell population size was measured following immunologic stimuli, which were expected to trigger antigen-specific T cells. RESULTS: Cellular microchimerism was found in SSc patients and controls, but the absolute amount of male DNA was higher in the patients, and the in vitro addition to blood mononuclear cells of an anti-CD28 costimulatory signal acted as a powerful amplification of microchimeric cells in 3 patients with SSc but not in controls. CONCLUSION: Cellular microchimerism is a physiologic phenomenon in parous women. In SSc patients, cellular microchimerism is accounted for by a higher number of cells that have the characteristics of T lymphocytes specific to maternal allogeneic antigens.
OBJECTIVE: To quantitatively measure male DNA in blood from women with systemic sclerosis (SSc) and from controls and to evaluate in vitro the modulation of the microchimeric cell population size following immunologic stimuli that were expected to trigger antigen-specific T cells. METHODS: A real-time polymerase chain reaction for a Y chromosome sequence was used to measure male DNA in blood from women with SSc and from controls who gave birth to sons. The in vitro change in the microchimeric cell population size was measured following immunologic stimuli, which were expected to trigger antigen-specific T cells. RESULTS: Cellular microchimerism was found in SSc patients and controls, but the absolute amount of male DNA was higher in the patients, and the in vitro addition to blood mononuclear cells of an anti-CD28 costimulatory signal acted as a powerful amplification of microchimeric cells in 3 patients with SSc but not in controls. CONCLUSION: Cellular microchimerism is a physiologic phenomenon in parous women. In SSc patients, cellular microchimerism is accounted for by a higher number of cells that have the characteristics of T lymphocytes specific to maternal allogeneic antigens.
Authors: Julie Di Cristofaro; Karlin R Karlmark; Sami B Kanaan; Doua F Azzouz; Marina El Haddad; Lucas Hubert; Dominique Farge-Bancel; Brigitte Granel; Jean Robert Harlé; Eric Hachulla; Etienne Pardoux; Jean Roudier; Christophe Picard; Nathalie C Lambert Journal: Front Immunol Date: 2018-08-14 Impact factor: 7.561
Authors: Blanca Cómitre-Mariano; Magdalena Martínez-García; Bárbara García-Gálvez; María Paternina-Die; Manuel Desco; Susanna Carmona; María Victoria Gómez-Gaviro Journal: iScience Date: 2021-12-29