Fate mapping of the larval silk glands of Bombyx mori by UV laser irradiation of the egg at fertilisation.

Localised UV laser irradiation of Bombyx eggs at the fertilisation stage causes localised cuticle defects in the integument of the resultant larvae. Based on the correlation between the site of irradiation and the site of defects, I previously established a fate map of the larval integument of Bombyx mori. Although most of the internal organs of the irradiated specimens were eliminated in the acidic fixative used for cuticle preparation, silk proteins which had accumulated in the gland lumen were resistant to the acid treatment and silk gland morphology was thus preserved. Taking advantage of this phenomenon, I continued fate mapping, now of the larval silk glands of Bombyx mori, utilising the same specimens that were prepared and analysed in the previous study. Of 710 irradiated specimens analysed, 452 showed two normal glands, 27 showed one normal and one deformed gland, 21 showed one normal gland only, and 210 showed no silk gland. The sites of irradiation that caused the deletion of a silk gland were located within a limited area that entirely overlapped the ventral half of the area where irradiation caused defects in the labium. These results are consistent with previous histological descriptions stating that the silk glands develop from the paired invaginations in the labial segment. Based on the data, the presumptive region for the larval silk glands was located on the previously established Bombyx fate map.