Literature DB >> 12682172

Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry.

T G M Bauters1, D Swinne, V Stove, H J Nelis.   

Abstract

A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.

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Year:  2003        PMID： 12682172      PMCID： PMC153898          DOI： 10.1128/JCM.41.4.1736-1737.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Rapid detection of single cell bacteria as a novel approach in food microbiology.

Authors:  Eva D'Haese; Hans J Nelis
Journal:  J AOAC Int       Date:  2002 Jul-Aug       Impact factor: 1.913

2.  Solid phase cytometry for detection of rare events.

Authors:  K Mignon-Godefroy; J G Guillet; C Butor
Journal:  Cytometry       Date:  1997-04-01

3.  Colonization of a voice prosthesis by Cryptococcus neoformans.

Authors:  T G Bauters; M Moerman; G Pini; H Vermeersch; H J Nelis
Journal:  Med Mycol       Date:  2001-08       Impact factor: 4.076

4.  Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays.

Authors:  Ralf Bialek; Michael Weiss; Kubrom Bekure-Nemariam; Laura K Najvar; Maria B Alberdi; John R Graybill; Udo Reischl
Journal:  Clin Diagn Lab Immunol       Date:  2002-03

5.  Serotyping of Cryptococcus neoformans by using a monoclonal antibody specific for capsular polysaccharide.

Authors:  F Dromer; E Gueho; O Ronin; B Dupont
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948
  5 in total
  3 in total

1.  Detection of bacteria in red blood cell concentrates by the Scansystem method.

Authors:  S Ribault; A Faucon; L Grave; P Nannini; I Besson Faure
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

2.  Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry.

Authors:  Lies M E Vanhee; Hans J Nelis; Tom Coenye
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

3.  Rapid screening method for detection of bacteria in platelet concentrates.

Authors:  S Ribault; K Harper; L Grave; C Lafontaine; P Nannini; A Raimondo; I Besson Faure
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948
  3 in total

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