Literature DB >> 12682163

Detection of hepatitis B virus DNA in sera from patients with chronic hepatitis B virus infection by DNA microarray method.

Kazunori Kawaguchi1, Shuichi Kaneko, Masao Honda, Hiroshi F Kawai, Yukihiro Shirota, Kenichi Kobayashi.   

Abstract

We have developed a sensitive and quantitative assay using a DNA microarray for the detection of hepatitis B virus (HBV) DNA in serum. Fluorescently labeled target cDNA prepared from cloned HBV DNA or serum HBV DNA was hybridized to capture DNA on a slide. A linear relationship was obtained between the intensities of the array spot and the amount of the cloned or serum HBV DNA, indicating the quantitative accuracy of this assay system. In addition, there was a significant correlation between the number of molecules of serum HBV DNA determined by the DNA microarray and that determined by a branched-DNA assay (n = 21, r = 0.89). Given these results, we conclude that the DNA microarray assay system may be useful as a diagnostic technique in the clinical laboratory.

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Year:  2003        PMID： 12682163      PMCID： PMC153866          DOI： 10.1128/JCM.41.4.1701-1704.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Genome-wide analysis of DNA copy-number changes using cDNA microarrays.

Authors:  J R Pollack; C M Perou; A A Alizadeh; M B Eisen; A Pergamenschikov; C F Williams; S S Jeffrey; D Botstein; P O Brown
Journal:  Nat Genet       Date:  1999-09       Impact factor: 38.330

2.  Universal DNA array detection of small insertions and deletions in BRCA1 and BRCA2.

Authors:  R Favis; J P Day; N P Gerry; C Phelan; S Narod; F Barany
Journal:  Nat Biotechnol       Date:  2000-05       Impact factor: 54.908

3.  Simple procedure of DNA isolation from human serum.

Authors:  M Ishizawa; Y Kobayashi; T Miyamura; S Matsuura
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

4.  Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses.

Authors:  M S Urdea; T Horn; T J Fultz; M Anderson; J A Running; S Hamren; D Ahle; C A Chang
Journal:  Nucleic Acids Symp Ser       Date:  1991

5.  Cell cycle regulation of hepatitis C virus internal ribosomal entry site-directed translation.

Authors:  M Honda; S Kaneko; E Matsushita; K Kobayashi; G A Abell; S M Lemon
Journal:  Gastroenterology       Date:  2000-01       Impact factor: 22.682

6.  Spot-blot hybridization assay for the detection of hepatitis B virus DNA in serum: factors determining its sensitivity and specificity.

Authors:  E Walter; H E Blum; W B Offensperger; C Zeschnigk; S Offensperger; W Gerok
Journal:  Hepatology       Date:  1987 May-Jun       Impact factor: 17.425

7.  Quantitative monitoring of gene expression patterns with a complementary DNA microarray.

Authors:  M Schena; D Shalon; R W Davis; P O Brown
Journal:  Science       Date:  1995-10-20       Impact factor: 47.728

8.  Cloning and structural analyses of hepatitis B virus DNAs, subtype adr.

Authors:  A Fujiyama; A Miyanohara; C Nozaki; T Yoneyama; N Ohtomo; K Matsubara
Journal:  Nucleic Acids Res       Date:  1983-07-11       Impact factor: 16.971

9.  An assay for the detection of the DNA genome of hepatitis B virus in serum.

Authors:  M Berninger; M Hammer; B Hoyer; J L Gerin
Journal:  J Med Virol       Date:  1982       Impact factor: 2.327

10.  Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique.

Authors:  S Kaneko; S M Feinstone; R H Miller
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948
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  8 in total

1.  Differentiation of the seven major lyssavirus species by oligonucleotide microarray.

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Journal:  J Clin Microbiol       Date:  2011-12-21       Impact factor: 5.948

2.  Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes.

Authors:  Shinjiro Honma; Vladimir Chizhikov; Norma Santos; Masatoshi Tatsumi; Maria do Carmo S T Timenetsky; Alexandre C Linhares; Joana D'Arc P Mascarenhas; Hiroshi Ushijima; George E Armah; Jon R Gentsch; Yasutaka Hoshino
Journal:  J Clin Microbiol       Date:  2007-06-13       Impact factor: 5.948

3.  Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants.

Authors:  Louis Davignon; Elizabeth A Walter; Kate M Mueller; Christopher P Barrozo; David A Stenger; Baochuan Lin
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

4.  Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses.

Authors:  Baochuan Lin; Gary J Vora; Dzung Thach; Elizabeth Walter; David Metzgar; Clark Tibbetts; David A Stenger
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

5.  Expression of NGF family and their receptors in gastric carcinoma: a cDNA microarray study.

Authors:  Jian-Jun Du; Ke-Feng Dou; Shu-You Peng; Bing-Zhi Qian; Hua-Sheng Xiao; Feng Liu; Wei-Zhong Wang; Wen-Xian Guan; Zhi-Qing Gao; Ying-Bin Liu; Ze-Guang Han
Journal:  World J Gastroenterol       Date:  2003-07       Impact factor: 5.742

6.  Use of the DNA flow-thru chip, a three-dimensional biochip, for typing and subtyping of influenza viruses.

Authors:  Nicole Kessler; Olivier Ferraris; Kevin Palmer; Wayne Marsh; Adam Steel
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

7.  Comprehensive detection and identification of seven animal coronaviruses and human respiratory coronavirus 229E with a microarray hybridization assay.

Authors:  Qin Chen; Jian Li; Zhirui Deng; Wei Xiong; Quan Wang; Yong-Qiang Hu
Journal:  Intervirology       Date:  2009-12-03       Impact factor: 1.763

Review 8.  Recombinant viral proteins for use in diagnostic ELISAs to detect virus infection.

Authors:  Kelly-Anne Spencer; Fernando A Osorio; Julian A Hiscox
Journal:  Vaccine       Date:  2007-03-06       Impact factor: 3.641
  8 in total

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