| Literature DB >> 12680755 |
Abstract
Employing atomic force microscopy as an in situ molecular force probe, we have measured the binding strength between cholera toxin B-pentamer (ctB) and its membrane receptor, ganglioside G(M1). By application of the basic principle of the reaction rate theory, key parameters of the ligand-receptor binding potential can be obtained from our data. The potential has a well with a spatial span of about 2.5 A and a depth of at least six times the thermal energy at room temperature. The very short range nature of the binding potential leads to the specificity of the ctB-G(M1) coupling.Entities:
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Year: 2003 PMID: 12680755 DOI: 10.1021/bi027016h
Source DB: PubMed Journal: Biochemistry ISSN: 0006-2960 Impact factor: 3.162