Hong Shen1, Guo-Jiang Huang, Yue-Wen Gong. 1. John Buhler Research Centre, 803G - 715 McDermot Avenue, Winnipeg, Manitoba, Canada, R3E 3P4. ygong@ms.umanitoba.ca
Abstract
AIM: To explore different roles of TGF-beta (transforming growth factor beta) and bone morphogenetic proteins (BMPs) in hepatic stellate cell proliferation and trans-differentiation. METHODS: Hepatic stellate cells were isolated from male Sprague-Dawley rats. Sub-cultured hepatic stellate cells were employed for cell proliferation assay with WST-1 reagent and Western blot analysis with antibody against smooth muscle alpha actin (SMA). RESULTS: The results indicated that TGF-beta1 significantly inhibited cell proliferation at concentration as low as 0.1 ng/ml, but both BMP-2 and BMP-4 did not affect cell proliferation at concentration as high as 10 ng/ml. The effect on hepatic stellate cell trans-differentiation was similar between TGF-beta1 and BMPs. However, BMPs was more potent at trans-differentiation of hepatic stellate cells than TGF-beta1. In addition, we observed that TGF-beta1 transient reduced the abundance of SMA in hepatic stellate cells. CONCLUSION: TGF-beta may be more important in regulation of hepatic stellate cell proliferation while BMPs may be the major cytokines regulating hepatic stellate cell trans-differentiation.
AIM: To explore different roles of TGF-beta (transforming growth factor beta) and bone morphogenetic proteins (BMPs) in hepatic stellate cell proliferation and trans-differentiation. METHODS: Hepatic stellate cells were isolated from male Sprague-Dawley rats. Sub-cultured hepatic stellate cells were employed for cell proliferation assay with WST-1 reagent and Western blot analysis with antibody against smooth muscle alpha actin (SMA). RESULTS: The results indicated that TGF-beta1 significantly inhibited cell proliferation at concentration as low as 0.1 ng/ml, but both BMP-2 and BMP-4 did not affect cell proliferation at concentration as high as 10 ng/ml. The effect on hepatic stellate cell trans-differentiation was similar between TGF-beta1 and BMPs. However, BMPs was more potent at trans-differentiation of hepatic stellate cells than TGF-beta1. In addition, we observed that TGF-beta1 transient reduced the abundance of SMA in hepatic stellate cells. CONCLUSION:TGF-beta may be more important in regulation of hepatic stellate cell proliferation while BMPs may be the major cytokines regulating hepatic stellate cell trans-differentiation.
Authors: Miguel Á Manzanares; Akihiro Usui; Deanna J Campbell; Catherine I Dumur; Gabrielle T Maldonado; Michel Fausther; Jonathan A Dranoff; Alphonse E Sirica Journal: Am J Pathol Date: 2017-03-15 Impact factor: 4.307
Authors: Carlos Ernesto Fernández-García; Stephania C Isaza; Esther Rey; Patricia Marañón; Rocío Gallego-Durán; Rocío Montero-Vallejo; Javier Rodríguez de Cía; Javier Ampuero; Manuel Romero-Gómez; Carmelo García-Monzón; Águeda González-Rodríguez Journal: Biomark Res Date: 2022-05-25