Literature DB >> 12679858

DNA homoduplexes containing no pyrimidine nucleotide.

Jaroslav Kypr1, Iva Kejnovská, Michaela Vorlícková.   

Abstract

We show using polyacrylamide gel electrophoresis that guanine+adenine repeat strands of DNA associate into homoduplexes at neutral pH and moderate ionic strength. The homoduplexes melt in a cooperative way like the Watson-Crick duplex, although they contain no Watson-Crick base pair. Guanine is absolutely needed for the homoduplex formation and the homoduplex stability increases with the guanine content of the repeat. The present results have implications for the nature of the first replicators, as well as regarding forces stabilizing the duplexes of DNA.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12679858     DOI： 10.1007/s00249-003-0287-x

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  35 in total

1.  Efforts toward expansion of the genetic alphabet: replication of DNA with three base pairs.

Authors:  E L Tae; Y Wu; G Xia; P G Schultz; F E Romesberg
Journal:  J Am Chem Soc       Date:  2001-08-01       Impact factor: 15.419

2.  Unnatural base pairs for specific transcription.

Authors:  T Ohtsuki; M Kimoto; M Ishikawa; T Mitsui; I Hirao; S Yokoyama
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-24       Impact factor: 11.205

3.  Dimerization of the guanine-adenine repeat strands of DNA.

Authors:  M Vorlícková; I Kejnovská; J Kovanda; J Kypr
Journal:  Nucleic Acids Res       Date:  1999-01-15       Impact factor: 16.971

4.  The case for an ancestral genetic system involving simple analogues of the nucleotides.

Authors:  G F Joyce; A W Schwartz; S L Miller; L E Orgel
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

5.  Prebiotic cytosine synthesis: a critical analysis and implications for the origin of life.

Authors:  R Shapiro
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-13       Impact factor: 11.205

6.  Significance of nucleobase shape complementarity and hydrogen bonding in the formation and stability of the closed polymerase-DNA complex.

Authors:  L Dzantiev; Y O Alekseyev; J C Morales; E T Kool; L J Romano
Journal:  Biochemistry       Date:  2001-03-13       Impact factor: 3.162

7.  Structure and Base Pairing Properties of a Replicable Nonpolar Isostere for Deoxyadenosine.

Authors:  Kevin M Guckian; Juan C Morales; Eric T Kool
Journal:  J Org Chem       Date:  1998       Impact factor: 4.354

8.  Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA.

Authors:  Jaroslav Kypr; Michaela Vorlícková
Journal:  Biopolymers       Date:  2002       Impact factor: 2.505

9.  The prebiotic role of adenine: a critical analysis.

Authors:  R Shapiro
Journal:  Orig Life Evol Biosph       Date:  1995-06       Impact factor: 1.950

10.  Alternating d(GA)n DNA sequences form antiparallel stranded homoduplexes stabilized by the formation of G.A base pairs.

Authors:  D Huertas; L Bellsolell; J M Casasnovas; M Coll; F Azorín
Journal:  EMBO J       Date:  1993-10       Impact factor: 11.598
View more
  1 in total

1.  Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule.

Authors:  Cheng-Chung Chang; Chih-Wei Chien; Yi-Hsueh Lin; Chi-Chih Kang; Ta-Chau Chang
Journal:  Nucleic Acids Res       Date:  2007-04-11       Impact factor: 16.971
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.