| Literature DB >> 12675592 |
Kalyani Mondal1, Aparna Sharma, Lata Lata, Munishwar Nath Gupta.
Abstract
The crude extracts of alpha-amylases when mixed with alginate, tert-butyl alcohol, and ammonium sulfate resulted in an interfacial precipitate containing polymer-bound amylase. The precipitate was dissolved in 1 M maltose to recover alpha-amylase activity. The recovery of alpha-amylases were 74%, 77%, and 92% in the case of Bacillus amyloliquefaciens, wheat germ, and porcine pancreas, respectively. All purified preparations showed a single band on SDS-PAGE.Entities:
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Year: 2003 PMID： 12675592 DOI： 10.1021/bp025619e
Source DB: PubMed Journal: Biotechnol Prog ISSN： 1520-6033