BACKGROUND: Intraoperative assessment of intestinal viability following release of strangulation remains difficult. The aim of this study was to establish clinical standards for the evaluation of intestinal viability by charge-coupled device (CCD) microscopy. METHODS: A rat ileus model with strangulation for between 15 and 120 min was used. The images obtained by CCD microscopy were used to calculate maximum velocity (V (max)) ratio (ratio of V (max) of blood cell transition in the experimental segment to that in the reference segment) and S ratio (the ratio of S-the effective area of the vascular bed against the total area of the vascular bed-in the experimental segment to that in the reference segment). Rats were divided into group 1, comprising animals that survived for 7 days or more, and group 2, which consisted of animals that died within 4 days. RESULTS: V (max) ratio and S ratio in group 1 were both significantly higher than those in group 2 (P < 0.01). No death occurred at a V (max) ratio of 0.76 or higher and an S ratio of 0.61 or more, while there were no survivors with a V (max) ratio of 0.54 or less and an S ratio of 0.51 or less. CONCLUSION: V (max) ratio and S ratio could be used as indices for evaluation of intestinal viability. Copyright 2003 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
BACKGROUND: Intraoperative assessment of intestinal viability following release of strangulation remains difficult. The aim of this study was to establish clinical standards for the evaluation of intestinal viability by charge-coupled device (CCD) microscopy. METHODS: A rat ileus model with strangulation for between 15 and 120 min was used. The images obtained by CCD microscopy were used to calculate maximum velocity (V (max)) ratio (ratio of V (max) of blood cell transition in the experimental segment to that in the reference segment) and S ratio (the ratio of S-the effective area of the vascular bed against the total area of the vascular bed-in the experimental segment to that in the reference segment). Rats were divided into group 1, comprising animals that survived for 7 days or more, and group 2, which consisted of animals that died within 4 days. RESULTS: V (max) ratio and S ratio in group 1 were both significantly higher than those in group 2 (P < 0.01). No death occurred at a V (max) ratio of 0.76 or higher and an S ratio of 0.61 or more, while there were no survivors with a V (max) ratio of 0.54 or less and an S ratio of 0.51 or less. CONCLUSION: V (max) ratio and S ratio could be used as indices for evaluation of intestinal viability. Copyright 2003 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
Authors: Michele Diana; Eric Noll; Anne-Laure Charles; Pierre Diemunsch; Bernard Geny; Yu-Yin Liu; Francesco Marchegiani; Luigi Schiraldi; Vincent Agnus; Veronique Lindner; Lee Swanström; Bernard Dallemagne; Jacques Marescaux Journal: Surg Endosc Date: 2016-06-20 Impact factor: 4.584
Authors: Michele Diana; Bernard Dallemagne; Hyunsoo Chung; Yoshihiro Nagao; Peter Halvax; Vincent Agnus; Luc Soler; Veronique Lindner; Nicolas Demartines; Pierre Diemunsch; Bernard Geny; Lee Swanström; Jacques Marescaux Journal: Surg Endosc Date: 2014-06-17 Impact factor: 4.584