| Literature DB >> 12669154 |
Antonio M Serafin1, John M Akudugu, Lothar Böhm.
Abstract
The relationship between radiosensitivity and DNA repair was investigated in six human prostate cell lines, 1542-NPTX, BPH-1, 1542-CP(3)TX, 1532-CP(2)TX, 1535-CP(1)TX and LNCaP. Except for LNCaP, these cell lines are new and were derived from primary prostate tumours and normal non-tumourigenic prostate tissue. Cell survival was assessed by clonogenic assay. DNA damage was determined in non-synchronised cells by constant-field gel electrophoresis, and expressed as the fraction of DNA released. For initial damage, cells were embedded in agarose and irradiated on ice with 0-100 Gy (60)Co gamma-irradiation. Residual DNA damage was measured after 2 h and 20 h of repair. Radiosensitivity, given as the mean inactivation dose, was found to vary between 1.62 and 2.77 Gy. We found that radiosensitivity significantly correlates with the 2 h DNA repair component, giving a correlation coefficient of 0.92 ( P=0.009). In the cell lines examined here the 2 h repair component emerges as an indicator of radiosensitivity.Entities:
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Year: 2003 PMID： 12669154 DOI： 10.1007/s00240-003-0299-0
Source DB: PubMed Journal: Urol Res ISSN： 0300-5623