| Literature DB >> 12667186 |
A M Alluwaimi1, C M Leutenegger, T B Farver, P V Rossitto, W L Smith, J S Cullor.
Abstract
TaqMan real time PCR was used to study the transcriptional activity of the bovine IL-2, IL-6, IL-12p40, IFN-gamma, TNF-alpha and granulocyte-monocyte colony stimulating factor of whole milk cells in bovine mammary gland experimentally infected with Staphylococcus aureus. Cytokine transcriptional activity was monitored at 7, 24 and 32 h Post-infection (Pi). IL-12 and TNF-alpha levels were significantly elevated at 24 h Pi followed by sharp decrease at 32 h pi. IL-2 level was decreased at 32 h pi. IL-12 and IFN-gamma showed a significant interaction at 24 h pi. The significant elevations of the IL-12 and TNF-alpha transcriptional level most likely indicate their important role in regulation of the immune responses of bovine mammary gland in S. aureus infection. Depression of IL-2 could reflect the suppressive nature of the S. aureus mastitis.Entities:
Mesh：
Substances：
Year: 2003 PMID： 12667186 DOI： 10.1046/j.1439-0450.2003.00628.x
Source DB: PubMed Journal: J Vet Med B Infect Dis Vet Public Health ISSN： 0931-1793