K A Derringer1, R W A Linden. 1. Department of Orthodontics, GKT Dental Institute, King's College, Caldecot Road, London SE5 9RQ, UK. kathryn.derringer@kcl.ac.uk
Abstract
OBJECTIVE: The aim of this study was to investigate if a combination of five diffusible angiogenic growth factors were released in human dental pulp during orthodontic force application, by using growth factor neutralising antibodies (NAs) to block their effects. MATERIALS AND METHODS: Dental pulps from 18 premolar teeth treated with orthodontic fixed appliances for 2 weeks were divided vertically, and each half pulp further sectioned and co-cultured with a section of rat aorta in collagen surrounded by growth media. NAs (anti-h VEGF, anti-h FGF2, anti-h PDGF, anti-TGFbeta and anti-h EGF) were added to the media of the co-cultures from one half of each pulp from 10 teeth. The co-cultures from the remaining eight teeth acted as controls. Sections of rat aorta alone were also cultured with and without NAs. Cultures were examined daily by light microscopy for growth and number of microvessels. RESULTS: NAs significantly reduced microvessel numbers in the co-cultures, but not in the rat aorta alone cultures. CONCLUSIONS: The results show that a combination of these angiogenic growth factors are released in the pulp following orthodontic force.
OBJECTIVE: The aim of this study was to investigate if a combination of five diffusible angiogenic growth factors were released in human dental pulp during orthodontic force application, by using growth factor neutralising antibodies (NAs) to block their effects. MATERIALS AND METHODS: Dental pulps from 18 premolar teeth treated with orthodontic fixed appliances for 2 weeks were divided vertically, and each half pulp further sectioned and co-cultured with a section of rat aorta in collagen surrounded by growth media. NAs (anti-h VEGF, anti-h FGF2, anti-h PDGF, anti-TGFbeta and anti-h EGF) were added to the media of the co-cultures from one half of each pulp from 10 teeth. The co-cultures from the remaining eight teeth acted as controls. Sections of rat aorta alone were also cultured with and without NAs. Cultures were examined daily by light microscopy for growth and number of microvessels. RESULTS: NAs significantly reduced microvessel numbers in the co-cultures, but not in the rat aorta alone cultures. CONCLUSIONS: The results show that a combination of these angiogenic growth factors are released in the pulp following orthodontic force.
Authors: Javier Caviedes-Bucheli; Luis F Lopez-Moncayo; Hernan Dario Muñoz-Alvear; Francisco Hernandez-Acosta; Melissa Pantoja-Mora; Angie S Rodriguez-Guerrero; Alexander López-Ordoñez; Luis E Díaz; Jose Francisco Gomez-Sosa; Hugo R Munoz Journal: BMC Oral Health Date: 2020-11-12 Impact factor: 2.757
Authors: Javier Caviedes-Bucheli; Luis Fernando Lopez-Moncayo; Hernan Dario Muñoz-Alvear; Jose Francisco Gomez-Sosa; Luis Eduardo Diaz-Barrera; Hernando Curtidor; Hugo Roberto Munoz Journal: BMC Oral Health Date: 2021-03-23 Impact factor: 2.757