Literature DB >> 12658787

Experimental study of plasmid TGF-beta 1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cells in vitro.

Qiong Huang1, Yanhua Hu, Fagang Jiang, Hong Chen.   

Abstract

To investigate whether the TGF-beta 1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-beta 1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-beta 1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-beta 1 protein expression specific for pMAMTGF-beta 1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-beta 1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.

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Year:  2002        PMID： 12658787     DOI： 10.1007/BF02904792

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  15 in total

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3.  A nonviral vector system for efficient gene transfer to corneal endothelial cells via membrane integrins.

Authors:  L Shewring; L Collins; S L Lightman; S Hart; K Gustafsson; J W Fabre
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4.  Targeted gene transfer to corneal endothelium in vivo by electric pulse.

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Authors:  J G Smith; R L Walzem; J B German
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6.  Gene transfer with liposomes to the intraocular tissues by different routes of administration.

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7.  Retroviral vector-mediated gene transfer into keratocytes in vitro and in vivo.

Authors:  B Seitz; L Moreira; E Baktanian; D Sanchez; B Gray; E M Gordon; W F Anderson; P J McDonnell
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8.  Ex vivo transduction of corneal epithelial progenitor cells using a retroviral vector.

Authors:  J J Bradshaw; W F Obritsch; B J Cho; D S Gregerson; E J Holland
Journal:  Invest Ophthalmol Vis Sci       Date:  1999-01       Impact factor: 4.799

9.  Adenovirus-mediated gene transfer to the ocular surface epithelium.

Authors:  K Tsubota; H Inoue; K Ando; M Ono; K Yoshino; I Saito
Journal:  Exp Eye Res       Date:  1998-11       Impact factor: 3.467

Review 10.  Cationic liposomes for direct gene transfer in therapy of cancer and other diseases.

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