| Literature DB >> 12656988 |
Ayano Satoh1, Yanzhuang Wang, Jörg Malsam, Matthew B Beard, Graham Warren.
Abstract
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.Entities:
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Year: 2003 PMID： 12656988 PMCID： PMC3282115 DOI： 10.1034/j.1600-0854.2003.00103.x
Source DB: PubMed Journal: Traffic ISSN： 1398-9219 Impact factor: 6.215