Literature DB >> 12656186

Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases.

Philip Hugenholtz1, Thomas Huber1.   

Abstract

A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.

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Year:  2003        PMID： 12656186     DOI： 10.1099/ijs.0.02441-0

Source DB:  PubMed          Journal:  Int J Syst Evol Microbiol        ISSN： 1466-5026            Impact factor:   2.747


  72 in total

1.  Spatial heterogeneity of crenarchaeal assemblages within mesophilic soil ecosystems as revealed by PCR-single-stranded conformation polymorphism profiling.

Authors:  Marek K Sliwinski; Robert M Goodman
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

2.  Genetic diversity of microbial eukaryotes in anoxic sediment around fumaroles on a submarine caldera floor based on the small-subunit rDNA phylogeny.

Authors:  Kiyotaka Takishita; Hiroshi Miyake; Masaru Kawato; Tadashi Maruyama
Journal:  Extremophiles       Date:  2005-03-03       Impact factor: 2.395

3.  Diversity, localization, and physiological properties of filamentous microbes belonging to Chloroflexi subphylum I in mesophilic and thermophilic methanogenic sludge granules.

Authors:  Takeshi Yamada; Yuji Sekiguchi; Hiroyuki Imachi; Yoichi Kamagata; Akiyoshi Ohashi; Hideki Harada
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

4.  Phylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometer-pore-size filters.

Authors:  Tatsuo Miyoshi; Teruki Iwatsuki; Takeshi Naganuma
Journal:  Appl Environ Microbiol       Date:  2005-02       Impact factor: 4.792

5.  Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques.

Authors:  B V Kjellerup; R H Veeh; P Sumithraratne; T R Thomsen; K Buckingham-Meyer; B Frølund; P Sturman
Journal:  J Ind Microbiol Biotechnol       Date:  2005-04-21       Impact factor: 3.346

Review 6.  Fate of heterotrophic microbes in pelagic habitats: focus on populations.

Authors:  Jakob Pernthaler; Rudolf Amann
Journal:  Microbiol Mol Biol Rev       Date:  2005-09       Impact factor: 11.056

7.  PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.

Authors:  Silvia G Acinas; Ramahi Sarma-Rupavtarm; Vanja Klepac-Ceraj; Martin F Polz
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

8.  Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat.

Authors:  Ruth E Ley; J Kirk Harris; Joshua Wilcox; John R Spear; Scott R Miller; Brad M Bebout; Julia A Maresca; Donald A Bryant; Mitchell L Sogin; Norman R Pace
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

9.  Development of a dinoflagellate-oriented PCR primer set leads to detection of picoplanktonic dinoflagellates from Long Island Sound.

Authors:  Senjie Lin; Huan Zhang; Yubo Hou; Lilibeth Miranda; Debashish Bhattacharya
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

10.  Bifidobacteria in feces and environmental waters.

Authors:  Regina Lamendella; Jorge W Santo Domingo; Catherine Kelty; Daniel B Oerther
Journal:  Appl Environ Microbiol       Date:  2007-11-09       Impact factor: 4.792
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