BACKGROUND: Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers. METHODS: Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies. RESULTS: All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells. CONCLUSIONS: The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
BACKGROUND: Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers. METHODS: Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies. RESULTS: All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells. CONCLUSIONS: The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
Authors: Martin Prádný; Jirí Michálek; Petr Lesný; Ales Hejcl; Jirí Vacík; Miroslav Slouf; Eva Syková Journal: J Mater Sci Mater Med Date: 2006-12 Impact factor: 3.896
Authors: Jirí Vacík; Barbora Dvoránková; Jirí Michálek; Martin Prádný; Eva Krumbholcová; Tatána Fenclová; Karel Smetana Journal: J Mater Sci Mater Med Date: 2007-08-01 Impact factor: 3.896
Authors: Lukas Lacina; Jan Plzak; Ondrej Kodet; Pavol Szabo; Martin Chovanec; Barbora Dvorankova; Karel Smetana Journal: Int J Mol Sci Date: 2015-10-12 Impact factor: 5.923