Literature DB >> 12652593

Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis.

Patrick Giavalisco1, Eckhard Nordhoff, Hans Lehrach, Johan Gobom, Joachim Klose.   

Abstract

To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.

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Year:  2003        PMID： 12652593     DOI： 10.1002/elps.200390016

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  16 in total

1.  High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome.

Authors:  Patrick Giavalisco; Daniel Wilson; Thomas Kreitler; Hans Lehrach; Joachim Klose; Johan Gobom; Paola Fucini
Journal:  Plant Mol Biol       Date:  2005-03       Impact factor: 4.076

2.  An Optimized Trichloroacetic Acid/Acetone Precipitation Method for Two-Dimensional Gel Electrophoresis Analysis of Qinchuan Cattle Longissimus Dorsi Muscle Containing High Proportion of Marbling.

Authors:  Ruijie Hao; Camus Adoligbe; Bijie Jiang; Xianlin Zhao; Linsheng Gui; Kaixing Qu; Sen Wu; Linsen Zan
Journal:  PLoS One       Date:  2015-04-20       Impact factor: 3.240

3.  Dissecting the Molecular Regulation of Natural Variation in Growth and Senescence of Two Eutrema salsugineum Ecotypes.

Authors:  Fanhua Wang; Zhibin Sun; Min Zhu; Qikun Zhang; Yufei Sun; Wei Sun; Chunxia Wu; Tongtong Li; Yiwu Zhao; Changle Ma; Hui Zhang; Yanxiu Zhao; Zenglan Wang
Journal:  Int J Mol Sci       Date:  2022-05-30       Impact factor: 6.208

4.  Differential proteomic analysis of Arabidopsis thaliana genotypes exhibiting resistance or susceptibility to the insect herbivore, Plutella xylostella.

Authors:  Richard M Collins; Muhammed Afzal; Deborah A Ward; Mark C Prescott; Steven M Sait; Huw H Rees; A Brian Tomsett
Journal:  PLoS One       Date:  2010-04-08       Impact factor: 3.240

Review 5.  Complete solubilization of formalin-fixed, paraffin-embedded tissue may improve proteomic studies.

Authors:  Shan-Rong Shi; Clive R Taylor; Carol B Fowler; Jeffrey T Mason
Journal:  Proteomics Clin Appl       Date:  2013-03-06       Impact factor: 3.494

6.  Optimization of large gel 2D electrophoresis for proteomic studies of skeletal muscle.

Authors:  Patrick W Reed; Allison Densmore; Robert J Bloch
Journal:  Electrophoresis       Date:  2012-04       Impact factor: 3.535

7.  Novel Ser/Thr protein phosphatase 5 (PP5) regulated targets during DNA damage identified by proteomics analysis.

Authors:  Bryan M Ham; Hemalatha Jayachandran; Feng Yang; Navdeep Jaitly; Ashoka D Polpitiya; Matthew E Monroe; Ling Wang; Rui Zhao; Samuel O Purvine; Eric A Livesay; David G Camp; Sandra Rossie; Richard D Smith
Journal:  J Proteome Res       Date:  2010-02-05       Impact factor: 4.466

8.  A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots.

Authors:  Elisete Pains Rodrigues; Adalgisa Ribeiro Torres; Jesiane Stefânia da Silva Batista; Luciano Huergo; Mariangela Hungria
Journal:  Genet Mol Biol       Date:  2012-06       Impact factor: 1.771

9.  Analysis of xylem sap proteins from Brassica napus.

Authors:  Julia Kehr; Anja Buhtz; Patrick Giavalisco
Journal:  BMC Plant Biol       Date:  2005-06-21       Impact factor: 4.215

10.  Optimization of Protein Extraction for Lichen Thalli.

Authors:  Anna S Kondratiuk; Oleksiy M Savchuk; Jae-Seoun Hur
Journal:  Mycobiology       Date:  2015-06-30       Impact factor: 1.858
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