Light and electron microscopic observations on the carotid bodies of rats following adaptation to high altitude.

The carotid bodies of rats were investigated by light and electron microscopy following adaptation to a simulated altitude of 7000 m. Some animals were studied immediately after readaptation to sea-level, other groups up to 41 days later. The animals of the first group show enlarged glomera with dilated capillaries as reported earlier. The type-I-cells are large with a light staining cytoplasm. The lobular configuration of the glomus is lost and intracapillary platelet thrombosis are frequently found. Under these thrombi there is usually a chief cell degeneration with edema and vacuolisation. There is also a noticeable decrease in so-called catecholamine bodies and the remaining ones are usually arranged along the cell borders. Electron microscopically the intercapillary tissue reveals an increase in collagen material partially replanning lost chief cells. Essentially unaltered sustentacular cells frequently enclose with their processes only collagen bundles and nerve fibres. During 41 days following the readaptation there is a narrowing of capillaries and vacuolisation, swelling and thromboses are no longer observed. The amount of collagen, however, remains the same. Dense cored vesicles again increase in number and the chief cells show a prominent interdigitation. Light microscopically the restoration to normal appears to be almost complete. Fine structural analysis, however, shows an appearently permanent distortion in the composition and cellular arrangement of the glomus.