| Literature DB >> 12648815 |
Young Sun Lee1, Ok Kyung Han, Chan Woo Park, Seong Il Suh, Sang Woo Shin, Chae Ha Yang, Tae Won Jeon, Eun Sil Lee, Kwang Joong Kim, Seong Ho Kim, Wang Keun Yoo, Hyo Jung Kim.
Abstract
The present study was conducted to evaluate the immunomodulatory effect of aqueous-extracted Astragali radix (ARE) in methotrexate (MTX)-treated mouse spleen cells. In spleen cell proliferation assay, ARE enhanced mitogenic activity in the dose-response manner. We also investigated the effect of ARE on the reducing of immune suppression caused by MTX in mouse spleen cells. MTX decreased the spleen cell proliferation (IC(50):800 microg/ml). However, ARE significantly reduced the suppression of cell proliferation by MTX in mouse spleen cells. Immunomodulatory effect of ARE were further investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, we examined the expressions of various cytokines such as IL-6, IL-1alpha, IL-1beta, IL-12p40, GM-CSF and TNF. Enhancement of IL-1alpha and IL-12p40 mRNA expressions were shown in mouse spleen cells by ARE. In spite of MTX treatment, the expressions of IL-1alpha and IL-12p40 mRNA sustained in spleen cells. These data indicate that (1) ARE has a protective effect of immune suppression, and (2) the immunomodulatory effects of ARE may be, in part, associated with the expressions of IL-1alpha and IL-12p40 mRNA as well as the mitogenic effect on spleen cells. Copyright 2002 Elsevier Science Ireland Ltd.Entities:
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Year: 2003 PMID： 12648815 DOI： 10.1016/s0378-8741(02)00298-2
Source DB: PubMed Journal: J Ethnopharmacol ISSN： 0378-8741 Impact factor: 4.360