OBJECTIVE: beta-Catenin has been previously associated with oncogenic activity in human cancers. We evaluated whether beta-catenin also plays a role in papillary serous ovarian neoplasms. METHODS: Immunohistochemistry for beta-catenin was performed on the primary ovarian serous neoplasms of 105 women. Of these, 10 were low malignant potential (LMP) serous tumors, and 95 were serous cancers. Nuclear beta-catenin staining was correlated with grade of tumor and median survival. OVCAR-3, OVCA-420, OVCA-432, and MDAH-277-10c were evaluated for beta-catenin localization and transfected with a T-cell factor (TCF) responsive reporter to evaluate beta-catenin transcriptional activity. RESULTS: Of 105 serous tumors, 13 (12.3%) demonstrated beta-catenin nuclear staining. Eleven of 48 high-grade serous carcinomas (23.0%) demonstrated nuclear staining compared with 1 low-grade serous carcinoma (2.1%) (P = 0.006). One LMP tumor had nuclear staining. beta-Catenin nuclear localization was undetectable in the cell lines tested. Furthermore, transient transfection of the cell lines with a TCF-responsive reporter did not demonstrate significant constitutive transcriptional activation. CONCLUSIONS: We found a statistically significant correlation between beta-catenin nuclear localization and ovarian high-grade serous carcinomas. Thus, deregulation of beta-catenin may play a role in the pathogenesis of ovarian high-grade serous carcinomas in contrast to ovarian low-grade serous carcinomas and LMP serous tumors.
OBJECTIVE:beta-Catenin has been previously associated with oncogenic activity in humancancers. We evaluated whether beta-catenin also plays a role in papillary serous ovarian neoplasms. METHODS: Immunohistochemistry for beta-catenin was performed on the primary ovarian serous neoplasms of 105 women. Of these, 10 were low malignant potential (LMP) serous tumors, and 95 were serous cancers. Nuclear beta-catenin staining was correlated with grade of tumor and median survival. OVCAR-3, OVCA-420, OVCA-432, and MDAH-277-10c were evaluated for beta-catenin localization and transfected with a T-cell factor (TCF) responsive reporter to evaluate beta-catenin transcriptional activity. RESULTS: Of 105 serous tumors, 13 (12.3%) demonstrated beta-catenin nuclear staining. Eleven of 48 high-grade serous carcinomas (23.0%) demonstrated nuclear staining compared with 1 low-grade serous carcinoma (2.1%) (P = 0.006). One LMP tumor had nuclear staining. beta-Catenin nuclear localization was undetectable in the cell lines tested. Furthermore, transient transfection of the cell lines with a TCF-responsive reporter did not demonstrate significant constitutive transcriptional activation. CONCLUSIONS: We found a statistically significant correlation between beta-catenin nuclear localization and ovarian high-grade serous carcinomas. Thus, deregulation of beta-catenin may play a role in the pathogenesis of ovarian high-grade serous carcinomas in contrast to ovarian low-grade serous carcinomas and LMP serous tumors.
Authors: Shin Yoshioka; Mandy L King; Sophia Ran; Hiroshi Okuda; James A MacLean; Mary E McAsey; Norihiro Sugino; Laurent Brard; Kounosuke Watabe; Kanako Hayashi Journal: Mol Cancer Res Date: 2012-01-09 Impact factor: 5.852
Authors: Maria V Barbolina; Yiuying Liu; Hilal Gurler; Mijung Kim; Andre A Kajdacsy-Balla; Lisa Rooper; Jaclyn Shepard; Michael Weiss; Lonnie D Shea; Peter Penzes; Matthew J Ravosa; M Sharon Stack Journal: J Biol Chem Date: 2012-11-14 Impact factor: 5.157
Authors: K A Voutilainen; M A Anttila; S M Sillanpää; K M Ropponen; S V Saarikoski; M T Juhola; V-M Kosma Journal: J Clin Pathol Date: 2006-02-03 Impact factor: 3.411
Authors: Melissa A Merritt; Peter G Parsons; Tanya R Newton; Adam C Martyn; Penelope M Webb; Adèle C Green; David J Papadimos; Glen M Boyle Journal: BMC Cancer Date: 2009-10-23 Impact factor: 4.430