PROBLEM: This study aims to investigate and compare in vitro, immune cell production of the immunosuppresor cytokine interleukin-10 (IL-10) and the proinflammatory cytokine tumor necrosis factor (TNF-alpha) between normal pregnancy (NP) and preeclampsia. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from age-matched patients with preeclampsia and women with a NP (n=3/group) and cultured for 48 hr in the absence and presence of the mitogen phytohaemagluttanin (PHA, 1/100). The concentration of IL-10 and TNF-alpha in the culture medium (CM) was measured by ELISA. RESULTS: Stimulated PBMCs associated with preeclampsia had significantly less IL-10 in the CM compared to NP (347+/-39, preeclampsia vs. 689+/-128, NP; p<0.05) but no difference in TNF-alpha. There was no significant difference in IL-10 or TNF-alpha concentration in the CM of unstimulated PBMCs between preeclampsia and NP. CONCLUSIONS: These findings suggest that under in vitro stimulated conditions preeclampsia is associated with an abnormality characterized by a diminished ability of peripheral immune cells to produce the immunosupressor cytokine interleukin-10.
PROBLEM: This study aims to investigate and compare in vitro, immune cell production of the immunosuppresor cytokine interleukin-10 (IL-10) and the proinflammatory cytokine tumor necrosis factor (TNF-alpha) between normal pregnancy (NP) and preeclampsia. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from age-matched patients with preeclampsia and women with a NP (n=3/group) and cultured for 48 hr in the absence and presence of the mitogen phytohaemagluttanin (PHA, 1/100). The concentration of IL-10 and TNF-alpha in the culture medium (CM) was measured by ELISA. RESULTS: Stimulated PBMCs associated with preeclampsia had significantly less IL-10 in the CM compared to NP (347+/-39, preeclampsia vs. 689+/-128, NP; p<0.05) but no difference in TNF-alpha. There was no significant difference in IL-10 or TNF-alpha concentration in the CM of unstimulated PBMCs between preeclampsia and NP. CONCLUSIONS: These findings suggest that under in vitro stimulated conditions preeclampsia is associated with an abnormality characterized by a diminished ability of peripheral immune cells to produce the immunosupressor cytokine interleukin-10.
Authors: Mariana Leticia Matias; Mariana Romão; Ingrid Cristina Weel; Vanessa Rocha Ribeiro; Priscila Rezeck Nunes; Vera Therezinha Borges; João Pessoa Araújo; José Carlos Peraçoli; Leandro de Oliveira; Maria Terezinha Peraçoli Journal: PLoS One Date: 2015-06-08 Impact factor: 3.240
Authors: Christine L Chiu; Kristy L Jackson; Nerissa L Hearn; Nicole Steiner; Geoffrey A Head; Joanne M Lind Journal: BMC Med Genet Date: 2014-08-30 Impact factor: 2.103