Wen-Lei Cao1, Yi-Xin Wang, Zu-Qiong Xiang, Zheng Li. 1. Shanghai Institute of Andrology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China. caowenlei@hotmail.com
Abstract
AIM: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. METHODS: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. RESULTS: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1+/-3.2 and 243.0+/-21.6, respectively, while those after thawing were 23.2+/-2.5 and 105.7+/-28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. CONCLUSION: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.
AIM: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. METHODS: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. RESULTS: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1+/-3.2 and 243.0+/-21.6, respectively, while those after thawing were 23.2+/-2.5 and 105.7+/-28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. CONCLUSION:HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.
Authors: Bharat Singh Rathod; H C Nakhashi; Gyanendra Singh Sengar; T V Raja; Umesh Singh; Rajib Deb Journal: Trop Anim Health Prod Date: 2022-10-19 Impact factor: 1.893
Authors: Loredana Zilli; José Beirão; Roberta Schiavone; Maria Paz Herraez; Antonio Gnoni; Sebastiano Vilella Journal: PLoS One Date: 2014-06-18 Impact factor: 3.240