| Literature DB >> 12645823 |
B Freeman1, N Smith, C Curtis, L Huckett, J Mill, I W Craig.
Abstract
We provide details of an inexpensive and rapid method for extraction of DNA from buccal swabs (including samples received through the mail) and from a range of other tissue samples. The procedure we have developed provides amounts of DNA adequate for several thousand polymerase chain reactions (PCRs), and we have validated its potential for long-term storage. Samples stored for > 4 years are of comparable concentration and provide as robust PCR templates as those tested immediately after extraction. The availability of this technology is of considerable significance in planning DNA banks from population collections and cohorts.Mesh:
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Year: 2003 PMID: 12645823 DOI: 10.1023/a:1021055617738
Source DB: PubMed Journal: Behav Genet ISSN: 0001-8244 Impact factor: 2.805