Literature DB >> 12645665

Modified pulsed-field gel electrophoresis protocol for typing of enterococci.

Baharak Saeedi1, Anita Hällgren, Jon Jonasson, Lennart E Nilsson, Håkan Hanberger, Barbro Isaksson.   

Abstract

Controlling the spread of vancomycin-resistant enterococci (VRE) is an important task in hospital epidemiology. Pulsed-field gel electrophoresis (PFGE) has become the golden standard for molecular epidemiological characterisation of enterococcal isolates. For separation of DNA fragments by PFGE, different electrophoresis conditions have been recommended, but none of these protocols allows a satisfactory separation of both small and large DNA fragments of enterococci simultaneously. In this study we have speeded up the preparation of chromosomal DNA and defined new electrophoresis conditions that enhance separation of small and large DNA fragments for subtyping of enterococci with a 24 h PFGE.

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Year:  2002        PMID： 12645665     DOI： 10.1034/j.1600-0463.2002.1101205.x

Source DB:  PubMed          Journal:  APMIS        ISSN： 0903-4641            Impact factor:   3.205


  9 in total

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Journal:  Appl Environ Microbiol       Date:  2020-09-17       Impact factor: 4.792

2.  Epidemiology and molecular analysis of intestinal colonization by vancomycin-resistant enterococci in greek hospitals.

Authors:  Achilleas Gikas; Athanasia Christidou; Efstathia Scoulica; Paulos Nikolaidis; Athanasios Skoutelis; Stamatina Levidiotou; Sofia Kartali; Efstratios Maltezos; Simeon Metalidis; John Kioumis; George Haliotis; Sofia Dima; Maria Roumbelaki; Nikoleta Papageorgiou; Evangelos I Kritsotakis; Yannis Tselentis
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

3.  ICESluvan, a 94-kilobase mosaic integrative conjugative element conferring interspecies transfer of VanB-type glycopeptide resistance, a novel bacitracin resistance locus, and a toxin-antitoxin stabilization system.

Authors:  Eva K Bjørkeng; Erik Hjerde; Torunn Pedersen; Arnfinn Sundsfjord; Kristin Hegstad
Journal:  J Bacteriol       Date:  2013-09-27       Impact factor: 3.490

4.  A multicentre hospital outbreak in Sweden caused by introduction of a vanB2 transposon into a stably maintained pRUM-plasmid in an Enterococcus faecium ST192 clone.

Authors:  Audun Sivertsen; Hanna Billström; Öjar Melefors; Barbro Olsson Liljequist; Karin Tegmark Wisell; Måns Ullberg; Volkan Özenci; Arnfinn Sundsfjord; Kristin Hegstad
Journal:  PLoS One       Date:  2014-08-25       Impact factor: 3.240

5.  Comparison of Enterococcus faecium and Enterococcus faecalis Strains isolated from water and clinical samples: antimicrobial susceptibility and genetic relationships.

Authors:  Gonzalo Castillo-Rojas; Marisa Mazari-Hiríart; Sergio Ponce de León; Rosa I Amieva-Fernández; Raúl A Agis-Juárez; Johannes Huebner; Yolanda López-Vidal
Journal:  PLoS One       Date:  2013-04-01       Impact factor: 3.240

6.  Transfer of tetracycline resistance genes with aggregation substance in food-borne Enterococcus faecalis.

Authors:  Jong-Mi Choi; Gun-Jo Woo
Journal:  Curr Microbiol       Date:  2014-12-07       Impact factor: 2.188

7.  Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblings Heliconius erato phyllis caterpillars.

Authors:  Rosana Huff; Rebeca Inhoque Pereira; Caroline Pissetti; Aldo Mellender de Araújo; Pedro Alves d'Azevedo; Jeverson Frazzon; Ana Paula GuedesFrazzon
Journal:  PeerJ       Date:  2020-02-27       Impact factor: 2.984

8.  Mode and dynamics of vanA-type vancomycin resistance dissemination in Dutch hospitals.

Authors:  Sergio Arredondo-Alonso; Janetta Top; Jukka Corander; Rob J L Willems; Anita C Schürch
Journal:  Genome Med       Date:  2021-01-20       Impact factor: 11.117

9.  Clonal dissemination of vancomycin-resistant Enterococcus faecium ST412 in a Brazilian region.

Authors:  Geraldo da Silva Alves; Monalessa Fábia Pereira; Lais de Lima Bride; Ana Paula Ferreira Nunes; Ricardo Pinto Schuenck
Journal:  Braz J Infect Dis       Date:  2017-07-29       Impact factor: 3.257
  9 in total

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