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Literature DB >> 12643522

DTASelect and Contrast: tools for assembling and comparing protein identifications from shotgun proteomics.

David L Tabb¹, W Hayes McDonald, John R Yates.

Abstract

The components of complex peptide mixtures can be separated by liquid chromatography, fragmented by tandem mass spectrometry, and identified by the SEQUEST algorithm. Inferring a mixture's source proteins requires that the identified peptides be reassociated. This process becomes more challenging as the number of peptides increases. DTASelect, a new software package, assembles SEQUEST identifications and highlights the most significant matches. The accompanying Contrast tool compares DTASelect results from multiple experiments. The two programs improve the speed and precision of proteomic data analysis.

Mesh：

Substances：
Peptides
Proteins

Year: 2002 PMID： 12643522 PMCID： PMC2811961 DOI： 10.1021/pr015504q

Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466

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