| Literature DB >> 12642237 |
Yoshihiro Abiko1, Michiko Nishimura, Kaoru Kusano, Keisuke Nakashima, Kazuhiko Okumura, Toshiya Arakawa, Taishin Takuma, Itaru Mizoguchi, Tohru Kaku.
Abstract
We have examined the expression of MIP-3alpha/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3alpha/CCL20 is regulated by bacterial infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3alpha, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycler using the double-stranded DNA dye, SYBR Green I. Oral epithelial cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3alpha mRNA. The expression of MIP-3alpha was upregulated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-alpha. By in situ hybridization, the detectable MIP-3alpha expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3alpha contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC.Entities:
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Year: 2003 PMID： 12642237 DOI： 10.1016/s0003-9969(02)00167-x
Source DB: PubMed Journal: Arch Oral Biol ISSN： 0003-9969 Impact factor: 2.633