Literature DB >> 1264061

Micro-scale two-dimensional polyacrylamide gell electrophoresis of ribosomal proteins.

A Lin, E Collatz, I G Wool.   

Abstract

A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.5 to 1 mug of each protein is required, and the entire procedure, including electrophoresis in both dimensions, and staining and destaining can be completed in 6 to 7 hours.

Entities:  

Mesh：

Substances：

Year:  1976        PMID： 1264061     DOI： 10.1007/BF00277296

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  20 in total

1.  Group fractionation of eukaryotic ribosomal proteins.

Authors:  E Collatz; A Lin; G Stöffler; K Tsurugi; I G Wool
Journal:  J Biol Chem       Date:  1976-03-25       Impact factor: 5.157

2.  A new two-dimensional gel electrophoresis system for the analysis of complex protein mixtures: application to the ribosome of E. coli.

Authors:  U C Knopf; A Sommer; J Kenny; R R Traut
Journal:  Mol Biol Rep       Date:  1975-03       Impact factor: 2.316

3.  A method for changing the pH of gel strips in the two-dimensional gel electrophoresis of ribosomal proteins.

Authors:  S Avital; D Elson
Journal:  Anal Biochem       Date:  1974-01       Impact factor: 3.365

4.  Separation and radioautography of microgram quantities of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis.

Authors:  G A Howard; R R Traut
Journal:  FEBS Lett       Date:  1973-01-15       Impact factor: 4.124

5.  Immunological and electrophoretical comparison of ribosomal proteins from eight species belonging to Enterobacteriaceae.

Authors:  M Geisser; G W Tischendorf; G Stöffler; H G Wittmann
Journal:  Mol Gen Genet       Date:  1973-12-20

6.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

7.  Ribosomal proteins. II. A rapid micromethod for electrophoretic separation of Escherichia coli ribosomal protein on gelatinized cellulose acetate.

Authors:  G Stöffler
Journal:  Mol Gen Genet       Date:  1967

8.  Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.

Authors:  C C Sherton; I G Wool
Journal:  J Biol Chem       Date:  1972-07-25       Impact factor: 5.157

9.  Studies on proteins of animal ribosomes. 8. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins of rat liver.

Authors:  H Welfle; J Stahl; H Bielka
Journal:  Biochim Biophys Acta       Date:  1971-09-28

10.  The intracellular site of synthesis of mitochndrial ribosomal proteins in Neurospora crassa.

Authors:  P M Lizardi; D J Luck
Journal:  J Cell Biol       Date:  1972-07       Impact factor: 10.539
View more
  8 in total

1.  The stimulation of Escherichia coli stringent factor-dependent synthesis of guanosine 3',5'-polyphosphate [(p)ppGpp] by rat liver ribosomal proteins.

Authors:  S Fehr; A Lin; I G Wool; D Richter
Journal:  Mol Gen Genet       Date:  1979-11

2.  Enrichment of special Novikoff hepatoma and regenerating liver mRNA by hybridization to cDNA-cellulose.

Authors:  F W Hirsch; K N Nall; W H Spohn; H Busch
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

3.  High resolution mini-two-dimensional gel electrophoresis of yeast ribosomal proteins. A standard nomenclature for yeast ribosomal proteins.

Authors:  G H Bollen; W H Mager; R J Planta
Journal:  Mol Biol Rep       Date:  1981-11-30       Impact factor: 2.316

4.  Structural homology between Drosophila melanogaster and Escherichia coli acidic ribosomal proteins.

Authors:  W Y Chooi; L M Sabatini; M Macklin
Journal:  Biochem Genet       Date:  1984-08       Impact factor: 1.890

5.  Studies on the kinetic sequence of in vitro ribosome assembly using cibacron blue F3GA as a general assembly inhibitor.

Authors:  D Datta; L M Changchien; G R Craven
Journal:  Nucleic Acids Res       Date:  1986-05-27       Impact factor: 16.971

6.  Immunological evidence for structural homology between Drosophila melanogaster (S14), rabbit liver (S12), Saccharomyces cerevisiae (S25), Bacillus subtilis (S6), and Escherichia coli (S6) ribosomal proteins.

Authors:  W Y Chooi; E Otaka
Journal:  Mol Cell Biol       Date:  1984-11       Impact factor: 4.272

7.  Distinction between mouse DNA polymerases alpha and beta by tryptic peptide mapping.

Authors:  S R Planck; K Tanabe; S H Wilson
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971

8.  Homology between Drosophila melanogaster and Escherichia coli ribosomal proteins.

Authors:  L M Sabatini; M D Macklin; W Y Chooi
Journal:  Mol Gen Genet       Date:  1982
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.