Literature DB >> 12637087

Optimisation of PCR reactions using primer chessboarding.

Rory Gunson1, Graeme Gillespie, William F Carman.   

Abstract

In-house polymerase chain reaction (PCR) assays are now an integral part of the work of most diagnostic microbiological laboratories. Despite the availability of commercial reagent 'master-mixes' of some PCR reagents, the optimisation of primers still poses a significant problem. Here, we describe a simple method to assess the concentration of primer needed in single round, multiplex, nested and 'real-time' PCR procedures.

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Year:  2003        PMID： 12637087     DOI： 10.1016/s1386-6532(03)00006-4

Source DB:  PubMed          Journal:  J Clin Virol        ISSN： 1386-6532            Impact factor:   3.168


  9 in total

1.  Simultaneous detection of six diarrhea-causing bacterial pathogens with an in-house PCR-luminex assay.

Authors:  Jie Liu; Jean Gratz; Athanasia Maro; Happy Kumburu; Gibson Kibiki; Mami Taniuchi; Arif Mahmud Howlader; Shihab U Sobuz; Rashidul Haque; Kaisar A Talukder; Shahida Qureshi; Anita Zaidi; Doris M Haverstick; Eric R Houpt
Journal:  J Clin Microbiol       Date:  2011-11-09       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Simultaneous detection and quantitation of cytomegalovirus, Epstein-Barr virus, and adenovirus by use of real-time PCR and pooled standards.

Authors:  R N Gunson; A R Maclean; S J Shepherd; W F Carman
Journal:  J Clin Microbiol       Date:  2009-01-14       Impact factor: 5.948

4.  Gene Expression Profiling via Multigene Concatemers.

Authors:  Kai Jin; Xiaoli Zheng; Yuxian Xia
Journal:  PLoS One       Date:  2011-01-18       Impact factor: 3.240

5.  Evaluation of critical design parameters for RT-qPCR-based analysis of multiple dUTPase isoform genes in mice.

Authors:  Gergely A Rácz; Nikolett Nagy; Zoltán Gál; Tímea Pintér; László Hiripi; Beáta G Vértessy
Journal:  FEBS Open Bio       Date:  2019-05-29       Impact factor: 2.693

Review 6.  Practical experience of high throughput real time PCR in the routine diagnostic virology setting.

Authors:  R N Gunson; T C Collins; W F Carman
Journal:  J Clin Virol       Date:  2006-02-07       Impact factor: 3.168

7.  The validation of a real-time RT-PCR assay which detects influenza A and types simultaneously for influenza A H1N1 (2009) and oseltamivir-resistant (H275Y) influenza A H1N1 (2009).

Authors:  Susan Bennett; Rory N Gunson; Alasdair MacLean; Rhona Miller; William F Carman
Journal:  J Virol Methods       Date:  2010-10-14       Impact factor: 2.014

8.  The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples.

Authors:  Susan Bennett; Rory N Gunson
Journal:  J Virol Methods       Date:  2016-12-28       Impact factor: 2.014

9.  The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs.

Authors:  Susan Bennett; William F Carman; Rory N Gunson
Journal:  J Virol Methods       Date:  2012-09-18       Impact factor: 2.014
  9 in total

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