OBJECTIVE: The purpose of this study was to determine whether plasma from women with severe preeclampsia stimulates the production of endothelial cell interleukin-6 production and whether vitamin E could inhibit this process. STUDY DESIGN: Human umbilical vein endothelial cells were cultured in the presence of 5% plasma from women with severe preeclampsia (n = 12) or healthy pregnant women at term (n = 12), with or without 50 micromol/L vitamin E. Levels of interleukin-6 in plasma and human umbilical vein endothelial cell-conditioned media were measured by enzyme-linked immunosorbent assay. RESULTS: Interleukin-6 levels were elevated 5-fold in preeclamptic plasma compared with normal pregnant plasma (P <.05). Human umbilical vein endothelial cell interleukin-6 production was increased 25% by preeclamptic plasma compared with normal pregnant plasma (P <.005), and increased interleukin-6 production by preeclamptic plasma was inhibited by vitamin E. CONCLUSION: Endothelial cell activation by preeclamptic plasma stimulates interleukin-6 production, which is inhibited by vitamin E. These findings provide a potential cellular mechanism for the beneficial effects of antioxidant therapy in preeclampsia.
OBJECTIVE: The purpose of this study was to determine whether plasma from women with severe preeclampsia stimulates the production of endothelial cell interleukin-6 production and whether vitamin E could inhibit this process. STUDY DESIGN:Human umbilical vein endothelial cells were cultured in the presence of 5% plasma from women with severe preeclampsia (n = 12) or healthy pregnant women at term (n = 12), with or without 50 micromol/L vitamin E. Levels of interleukin-6 in plasma and human umbilical vein endothelial cell-conditioned media were measured by enzyme-linked immunosorbent assay. RESULTS:Interleukin-6 levels were elevated 5-fold in preeclamptic plasma compared with normal pregnant plasma (P <.05). Human umbilical vein endothelial cell interleukin-6 production was increased 25% by preeclamptic plasma compared with normal pregnant plasma (P <.005), and increased interleukin-6 production by preeclamptic plasma was inhibited by vitamin E. CONCLUSION: Endothelial cell activation by preeclamptic plasma stimulates interleukin-6 production, which is inhibited by vitamin E. These findings provide a potential cellular mechanism for the beneficial effects of antioxidant therapy in preeclampsia.
Authors: Charles J Lockwood; Chih-Feng Yen; Murat Basar; Umit A Kayisli; Maritza Martel; Irina Buhimschi; Catalin Buhimschi; S Joseph Huang; Graciela Krikun; Frederick Schatz Journal: Am J Pathol Date: 2008-05-08 Impact factor: 4.307
Authors: M L Martinez-Fierro; I Garza-Veloz; K Carrillo-Sanchez; V Martinez-Gaytan; R Cortes-Flores; M A Ochoa-Torres; G G Guerrero; I P Rodriguez-Sanchez; C O Cancela-Murrieta; M Zamudio-Osuna; J I Badillo-Almaraz; C Castruita-De la Rosa Journal: Hypertens Pregnancy Date: 2013-12-02 Impact factor: 2.108