Literature DB >> 12633907

Cloning and expression of the dihydroorotate dehydrogenase from Toxoplasma gondii.

Maria L Sierra Pagan1, Barbara H Zimmermann.   

Abstract

A full-length dihydroorotate dehydrogenase (DHODase) sequence was cloned from a Toxoplasma gondii tachyzoite cDNA library. The sequence was most similar to family 2 DHODases, and had a calculated molecular mass of 65.1 kDa. The full-length and two N-terminally truncated T. gondii DHODase sequences were expressed as recombinant proteins. One of the truncated sequences complemented a DHODase-deficient bacterial host.

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Year:  2003        PMID: 12633907     DOI: 10.1016/s0925-4439(02)00226-0

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  4 in total

Review 1.  Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.

Authors:  Mahmoud H El Kouni
Journal:  Comp Biochem Physiol B Biochem Mol Biol       Date:  2017-07-21       Impact factor: 2.231

Review 2.  Targeting purine and pyrimidine metabolism in human apicomplexan parasites.

Authors:  John E Hyde
Journal:  Curr Drug Targets       Date:  2007-01       Impact factor: 3.465

3.  Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii.

Authors:  Miryam Andrea Hortua Triana; My-Hang Huynh; Manuel F Garavito; Barbara A Fox; David J Bzik; Vern B Carruthers; Monika Löffler; Barbara H Zimmermann
Journal:  Mol Biochem Parasitol       Date:  2012-05-08       Impact factor: 1.759

4.  Babesia bovis dihydroorotate dehydrogenase (BboDHODH) is a novel molecular target of drug for bovine babesiosis.

Authors:  Ketsarin Kamyingkird; Shinuo Cao; Tatsunori Masatani; Paul Franck Adjou Moumouni; Patrick Vudriko; Ahmed Abd El Moniem Mousa; Mohamad Alaa Terkawi; Yoshifumi Nishikawa; Ikuo Igarashi; Xuenan Xuan
Journal:  J Vet Med Sci       Date:  2013-11-01       Impact factor: 1.267

  4 in total

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