Literature DB >> 12633851

Specific conjugation of DNA binding proteins to DNA templates through thiol-disulfide exchange.

Valeri G Metelev1, Elena A Kubareva, Olga V Vorob'eva, Andrey S Romanenkov, Tatiana S Oretskaya.   

Abstract

The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.

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Year:  2003        PMID: 12633851     DOI: 10.1016/s0014-5793(03)00122-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  1 in total

1.  Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases.

Authors:  Sandra Sagredo; Tobias Pirzer; Ali Aghebat Rafat; Marisa A Goetzfried; Gabriel Moncalian; Friedrich C Simmel; Fernando de la Cruz
Journal:  Angew Chem Int Ed Engl       Date:  2016-02-24       Impact factor: 15.336

  1 in total

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