AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province. METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers(PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics. RESULTS: Allele frequency (AF) of HLA-DRB1*0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs 0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1*0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles. CONCLUSION: HLA-DRB1*0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1*0901 may be susceptible to esophageal carcinoma.
AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province. METHODS:HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers(PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics. RESULTS: Allele frequency (AF) of HLA-DRB1*0901 was significantly higher in esophageal carcinomapatients than that in the normal controls (0.2500 vs 0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1*0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles. CONCLUSION:HLA-DRB1*0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1*0901 may be susceptible to esophageal carcinoma.
Authors: M A Chatzipetrou; K E Tarassi; M M Konstadoulakis; H E Pappas; K D Zafirellis; T E Athanassiades; S A Papadopoulos; D G Panousopoulos; B C Golematis; C A Papasteriades Journal: Dis Colon Rectum Date: 1999-01 Impact factor: 4.585
Authors: D Nagorsen; U Keilholz; L Rivoltini; A Schmittel; A Letsch; A M Asemissen; G Berger; H J Buhr; E Thiel; C Scheibenbogen Journal: Cancer Res Date: 2000-09-01 Impact factor: 12.701
Authors: C Zavaglia; M Martinetti; E Silini; R Bottelli; C Daielli; M Asti; A Airoldi; L Salvaneschi; M U Mondelli; G Ideo Journal: J Hepatol Date: 1998-01 Impact factor: 25.083
Authors: B G Weinshenker; P Santrach; A S Bissonet; S K McDonnell; D Schaid; S B Moore; M Rodriguez Journal: Neurology Date: 1998-09 Impact factor: 9.910