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Literature DB >> 12632171

Extracellular metalloproteinase activity in Phytomonas françai.

Flávia V S Almeida¹, Marta H Branquinha, Salvatore Giovanni-De-Simone, Alane B Vermelho.

Abstract

Extracellular proteolytic activities were detected in Phytomonas françai culture supernatant. A 67-kDa enzyme was purified by ammonium sulfate precipitation and gel filtration in a HPLC system. This proteinase was optimally active at 28 degrees C and pH 5.0; and the use of proteolytic inhibitors indicated that it belongs to the metalloproteinase class. This is the first report on the purification of an extracellular metalloproteinase from a Phytomonas species.

Entities: Chemical Species

Mesh：

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Year: 2002 PMID： 12632171 DOI： 10.1007/s00436-002-0792-3

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

11 in total

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8. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates.

Authors: C Heussen; E B Dowdle
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Extracellular metalloproteinase activity in Phytomonas françai.

1. Cell-associated and extracellular proteinases in Blastocrithidia culicis: influence of growth conditions.

Review 2. Phytomonas and other trypanosomatid parasites of plants and fruit.

3. Ubiquity of cysteine- and metalloproteinase activities in a wide range of trypanosomatids.

4. Crithidia guilhermei: purification and partial characterization of a 62-kDa extracellular metalloproteinase.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 6. Protein trafficking in kinetoplastid protozoa.

Review 7. The proteases and pathogenicity of parasitic protozoa.

8. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates.

9. Differential expression of proteolytic enzymes in endosymbiont-harboring Crithidia species.

10. Simplified endoproteinase assays using gelatin or azogelatin.

1. Gp63-like molecules in Phytomonas serpens: possible role in the insect interaction.

2. NADH-ubiquinone oxidoreductase activity in the kinetoplasts of the plant trypanosomatid Phytomonas serpens.

Review 3. Major surface protease of trypanosomatids: one size fits all?