Literature DB >> 1262786

Characterization of DNA excreted from phytohemagglutinin-stimulated lymphocytes.

J C Rogers.   

Abstract

The DNA released into the culture medium after phytohemagglutinin (PHA) stimulation of human peripheral blood lymphocytes has been purified and characterized. It is double stranded, sediments at 7-8S in alkaline sucrose, and has a Tm determined optically and by thermal elution from hydroxyapatite that is substantially lower than that found for lymphocyte cell DNA. Media DNA contains a major component reassociating with an average Cot-1/2 of 87 mol X s/liter, compared to a Cot-1/2 of 770 mol X s/liter for the unique fraction of cell DNA as measured by reassociation in 0.6 M Na+. This component of media DNA consists of unique sequence elements which are largely shared in media DNA preparations from cultures derived from different cell donors. The marked difference between media DNA and cell DNA indicates that media DNA is not derived from cell death and lysis, rather than some unique portion of lymphocyte DNA is apparently excreted from the cells during PHA-stimulated growth.

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Year:  1976        PMID： 1262786      PMCID： PMC2190176          DOI： 10.1084/jem.143.5.1249

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  22 in total

1.  Structural genes adjacent to interspersed repetitive DNA sequences.

Authors:  E H Davidson; B R Hough; W H Klein; R J Britten
Journal:  Cell       Date:  1975-03       Impact factor: 41.582

2.  Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system.

Authors:  P Anker; M Stroun; P A Maurice
Journal:  Cancer Res       Date:  1975-09       Impact factor: 12.701

3.  Cleavage of replicative intermediates of simian virus 40 deoxyribonucleic acid by the restriction endonuclease of Escherichia coli B.

Authors:  G C Fareed; E D Sebring; N P Salzman
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

4.  Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes.

Authors:  L D Gelb; D E Kohne; M A Martin
Journal:  J Mol Biol       Date:  1971-04-14       Impact factor: 5.469

5.  Reassociation and dissociation of cytoplasmic membrane-associated DNA.

Authors:  W Meinke; D A Goldstein
Journal:  J Mol Biol       Date:  1974-07-15       Impact factor: 5.469

6.  Uptake and release of DNA by lymphoid tissue and cells.

Authors:  I Olsen; G Harris
Journal:  Immunology       Date:  1974-12       Impact factor: 7.397

7.  Cell interactions in the immune response in vitro. V. Specific collaboration via complexes of antigen and thymus-derived cell immunoglobulin.

Authors:  M Feldmann
Journal:  J Exp Med       Date:  1972-10-01       Impact factor: 14.307

8.  Excretion of deoxyribonucleic acid by lymphocytes stimulated with phytohemagglutinin or antigen.

Authors:  J C Rogers; D Boldt; S Kornfeld; A Skinner; C R Valeri
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

9.  The response of human peripheral blood lymphocytes to phytohemagglutinin: determination of cell numbers.

Authors:  C C Stewart; S F Cramer; P G Steward
Journal:  Cell Immunol       Date:  1975-04       Impact factor: 4.868

10.  Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid.

Authors:  H T Tai; C A Smith; P A Sharp; J Vinograd
Journal:  J Virol       Date:  1972-02       Impact factor: 5.103
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  16 in total

1.  Identification of an intracellular precursor to DNA excreted by human lymphocytes.

Authors:  J C Rogers
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

2.  Selective release of excreted DNA sequences from phytohemagglutinin-stimulated human peripheral blood lymphocytes. Effects of trypsin and divalent cations.

Authors:  C W Distelhorst; K Cramer; J C Rogers
Journal:  J Clin Invest       Date:  1978-05       Impact factor: 14.808

3.  Kinetics of cell death and disintegration in human lymphocyte cultures.

Authors:  J L Bernheim; J Mendelsohn; M F Kelley; R Dorian
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

4.  Lactoferrin binds to cell membrane DNA. Association of surface DNA with an enriched population of B cells and monocytes.

Authors:  R M Bennett; J Davis; S Campbell; S Portnoff
Journal:  J Clin Invest       Date:  1983-03       Impact factor: 14.808

5.  Excretion of newly synthesized DNA by tonsil lymphocytes.

Authors:  M Staub; F Antoni
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

6.  Unstable high molecular weight inverted repetitive DNA in human lymphocytes.

Authors:  J C Rogers; T E Rucinsky
Journal:  Nucleic Acids Res       Date:  1982-09-25       Impact factor: 16.971

7.  Information carried by the DNA released by antigen-stimulated lymphocytes.

Authors:  D Jachertz; P Anker; P A Maurice; M Stroun
Journal:  Immunology       Date:  1979-08       Impact factor: 7.397

8.  Mechanisms of action of 'lymphocyte activating factor' (LAF) I. Association of lymphocyte activating factor action with early DNA synthesis in PHA-stimulated lymphocytes.

Authors:  F Kiertdzenbaum; B H Waksman
Journal:  Immunology       Date:  1977-11       Impact factor: 7.397

9.  Kinetics of thymidine incorporation into detergent-soluble DNA of mouse lymphocytes.

Authors:  P R Strauss; P T Banerjee; K LaGree; S C Mui
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

10.  The fate of concanavalin A-stimulated lymphocytes in the spleens of recipient mice.

Authors:  G Harris; A Martini; I Olsen
Journal:  Immunology       Date:  1978-08       Impact factor: 7.397
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