| Literature DB >> 12626405 |
Manfred Wuhrer1, Christiane Grimm, Ulrich Zahringer, Roger D Dennis, Clemens M Berkefeld, Mohamed A Idris, Rudolf Geyer.
Abstract
The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcalpha1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcalpha1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.Entities:
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Year: 2002 PMID： 12626405 DOI： 10.1093/glycob/cwg005
Source DB: PubMed Journal: Glycobiology ISSN： 0959-6658 Impact factor: 4.313