M V Palmer1, W Ray Waters, D L Whipple. 1. Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2300 Dayton Avenue, Ames, IA 50010, USA. mpalmer@nadc.ars.usda.gov
Abstract
SETTING: Although animal models of aerosol inoculation of Mycobacterium tuberculosis and M. bovis have been reported using laboratory animals, a model of aerosol delivery of M. bovis to cattle has not been reported previously. OBJECTIVE: Develop and characterize a model of aerosol delivery of M. bovis to cattle, and compare the distribution of lesions in cattle infected with either of two different strains of M. bovis, one isolated from cattle (HC2005T), and the other isolated from white-tailed deer (1315). DESIGN: Cattle (n=20, female and castrated males) aged 4 months, were infected with 1 x 10(3) (n=5) or 1 x 10(5) (n=5) colony-forming units (CFU) of M. bovis 1315 or 1 x 10(3) (n=5) or 1x10(5) (n=5) CFU of M. bovis HC2005T. Calves were infected using a commercially available aerosol delivery system. One hundred fifty-five days after infection, calves were euthanized, examined and tissues collected for microscopic analysis and bacteriologic culture. RESULTS: Nineteen of 20 calves developed tuberculosis. Typical tuberculous lesions were most pronounced in the lungs and tracheobronchial and mediastinal lymph nodes. CONCLUSION: The system described provides a reliable method of aerosol delivery of M. bovis to cattle. Lesion distribution suggests that the aerosolized inoculum was delivered deep into pulmonary alveoli and thus represents true aerosol exposure. Disease was more severe in groups receiving the highest dose of either inoculum strain; however, differences between strains were not seen. Published by Elsevier Science Ltd.
SETTING: Although animal models of aerosol inoculation of Mycobacterium tuberculosis and M. bovis have been reported using laboratory animals, a model of aerosol delivery of M. bovis to cattle has not been reported previously. OBJECTIVE: Develop and characterize a model of aerosol delivery of M. bovis to cattle, and compare the distribution of lesions in cattle infected with either of two different strains of M. bovis, one isolated from cattle (HC2005T), and the other isolated from white-tailed deer (1315). DESIGN:Cattle (n=20, female and castrated males) aged 4 months, were infected with 1 x 10(3) (n=5) or 1 x 10(5) (n=5) colony-forming units (CFU) of M. bovis 1315 or 1 x 10(3) (n=5) or 1x10(5) (n=5) CFU of M. bovis HC2005T. Calves were infected using a commercially available aerosol delivery system. One hundred fifty-five days after infection, calves were euthanized, examined and tissues collected for microscopic analysis and bacteriologic culture. RESULTS: Nineteen of 20 calves developed tuberculosis. Typical tuberculous lesions were most pronounced in the lungs and tracheobronchial and mediastinal lymph nodes. CONCLUSION: The system described provides a reliable method of aerosol delivery of M. bovis to cattle. Lesion distribution suggests that the aerosolized inoculum was delivered deep into pulmonary alveoli and thus represents true aerosol exposure. Disease was more severe in groups receiving the highest dose of either inoculum strain; however, differences between strains were not seen. Published by Elsevier Science Ltd.
Authors: Konstantin P Lyashchenko; Rena Greenwald; Alina Sikar-Gang; Archana A Sridhara; Ashley Johnathan; Paul Lambotte; Javan Esfandiari; Mayara F Maggioli; Tyler C Thacker; Mitchell V Palmer; W Ray Waters Journal: Clin Vaccine Immunol Date: 2017-06-05
Authors: W Ray Waters; Mitchell V Palmer; Molly R Stafne; Kristin E Bass; Mayara F Maggioli; Tyler C Thacker; Rick Linscott; John C Lawrence; Jeffrey T Nelson; Javan Esfandiari; Rena Greenwald; Konstantin P Lyashchenko Journal: Clin Vaccine Immunol Date: 2015-04-08
Authors: Hye Cheong Koo; Yong Ho Park; Jongsam Ahn; W Ray Waters; Mitch V Palmer; Mary Jo Hamilton; George Barrington; Abdelaziz A Mosaad; Kun Taek Park; Woo Kyung Jung; In Yeong Hwang; Sang-Nae Cho; Sang Jae Shin; William C Davis Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948
Authors: W R Waters; B M Buddle; H M Vordermeier; E Gormley; M V Palmer; T C Thacker; J P Bannantine; J R Stabel; R Linscott; E Martel; F Milian; W Foshaug; J C Lawrence Journal: Clin Vaccine Immunol Date: 2011-09-14
Authors: Lawrence R Green; Cynthia C Jones; Anne L Sherwood; Inna V Garkavi; Gerard A Cangelosi; Tyler C Thacker; Mitchell V Palmer; W Ray Waters; Chris V Rathe Journal: Clin Vaccine Immunol Date: 2009-07-15
Authors: W Ray Waters; Mitchell V Palmer; Brian J Nonnecke; Tyler C Thacker; Charles F Capinos Scherer; D Mark Estes; R Glyn Hewinson; H Martin Vordermeier; S Whitney Barnes; Glenn C Federe; John R Walker; Richard J Glynne; Tsungda Hsu; Brian Weinrick; Karolin Biermann; Michelle H Larsen; William R Jacobs Journal: Vaccine Date: 2009-01-09 Impact factor: 3.641
Authors: W Ray Waters; Mitchell V Palmer; Brian J Nonnecke; Tyler C Thacker; D Mark Estes; Michelle H Larsen; William R Jacobs; Peter Andersen; James McNair; F C Minion; Konstantin P Lyashchenko; R Glyn Hewinson; H Martin Vordermeier; Randy E Sacco Journal: PLoS One Date: 2009-07-29 Impact factor: 3.240