OBJECTIVE: To clarify whether female sex steroids directly affect the bioactivity of the human endometrial CD16neg CD56bright natural killer (NK) cells. DESIGN: In vitro study. SETTING: University obstetrics and gynecology department. PATIENT(S): Thirteen women with histologically normal endometrium who were undergoing hysterectomy and seven women during the first trimester of pregnancy who were undergoing selective termination. INTERVENTION(S): Endometrium or decidua was obtained. MAIN OUTCOME MEASURE(S): The effects of 17beta-estradiol or progesterone (10(-6), 10(-7), and 10(-8) M) on the proliferation, cytolytic activity, and cytokine secretion of the isolated endometrial CD16neg CD56bright NK cells were examined using a 3H-thymidine incorporation assay, 51Cr-releasing assay, and enzyme-linked immunosorbent assay, respectively. RESULT(S): Neither 17beta-estradiol nor progesterone had significant effects on the proliferation, cytolytic activity, and cytokine secretion of endometrial CD16neg CD56bright NK cells. CONCLUSION(S): Female sex steroids do not directly affect the bioactivity of the human endometrial CD16neg CD56bright NK cells.
OBJECTIVE: To clarify whether female sex steroids directly affect the bioactivity of the human endometrial CD16neg CD56bright natural killer (NK) cells. DESIGN: In vitro study. SETTING: University obstetrics and gynecology department. PATIENT(S): Thirteen women with histologically normal endometrium who were undergoing hysterectomy and seven women during the first trimester of pregnancy who were undergoing selective termination. INTERVENTION(S): Endometrium or decidua was obtained. MAIN OUTCOME MEASURE(S): The effects of 17beta-estradiol or progesterone (10(-6), 10(-7), and 10(-8) M) on the proliferation, cytolytic activity, and cytokine secretion of the isolated endometrial CD16neg CD56bright NK cells were examined using a 3H-thymidine incorporation assay, 51Cr-releasing assay, and enzyme-linked immunosorbent assay, respectively. RESULT(S): Neither 17beta-estradiol nor progesterone had significant effects on the proliferation, cytolytic activity, and cytokine secretion of endometrial CD16neg CD56bright NK cells. CONCLUSION(S): Female sex steroids do not directly affect the bioactivity of the human endometrial CD16neg CD56bright NK cells.
Authors: Marianne J van den Heuvel; Sirirak Chantakru; Xie Xuemei; Sharon S Evans; Francis Tekpetey; Patricia A Mote; Christine L Clarke; B Anne Croy Journal: Immunol Invest Date: 2005 Impact factor: 3.657
Authors: Satarupa Basu; Patricia A Pioli; Jose Conejo-Garcia; Charles R Wira; Charles L Sentman Journal: Clin Immunol Date: 2008-08-26 Impact factor: 3.969