BACKGROUND: Efficient killing of tumor cells depends on T cells that migrate from the circulation to the peripheral tissues; these cells express CD31. This study was undertaken to determine the impact of open (OS) and laparoscopic (LS) colorectal surgery on the percentage of circulating CD3+CD31+ cells. METHODS: Peripheral blood was collected from 27 OS and 24 LS colon cancer patients preoperatively (preOP) and on postoperative days 1 (POD1) and 3 (POD3). CD31+ T cells were assessed by flow cytometry using monoclonal antibodies. RESULTS: In the OS group, the percentage of CD3+CD31+ cells was significantly lower in POD1 and POD3 samples compared to the preOP results. LS surgery did not result in a significant change in the percentage of these T cells. A significant correlation was found between the decrease in the percentage of CD3+CD31+ cells and the length of incision in OS patients. CONCLUSIONS: The percentage of CD3+CD31+ cells decreases following OS but not LS and may be related to incision length. This may compromise T cell function in the peripheral tissues in the postoperative period.
BACKGROUND: Efficient killing of tumor cells depends on T cells that migrate from the circulation to the peripheral tissues; these cells express CD31. This study was undertaken to determine the impact of open (OS) and laparoscopic (LS) colorectal surgery on the percentage of circulating CD3+CD31+ cells. METHODS: Peripheral blood was collected from 27 OS and 24 LS colon cancerpatients preoperatively (preOP) and on postoperative days 1 (POD1) and 3 (POD3). CD31+ T cells were assessed by flow cytometry using monoclonal antibodies. RESULTS: In the OS group, the percentage of CD3+CD31+ cells was significantly lower in POD1 and POD3 samples compared to the preOP results. LS surgery did not result in a significant change in the percentage of these T cells. A significant correlation was found between the decrease in the percentage of CD3+CD31+ cells and the length of incision in OSpatients. CONCLUSIONS: The percentage of CD3+CD31+ cells decreases following OS but not LS and may be related to incision length. This may compromise T cell function in the peripheral tissues in the postoperative period.
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Authors: T Hensler; H Hecker; K Heeg; C D Heidecke; H Bartels; W Barthlen; H Wagner; J R Siewert; B Holzmann Journal: Infect Immun Date: 1997-06 Impact factor: 3.441
Authors: C G Elias; J P Spellberg; B Karan-Tamir; C H Lin; Y J Wang; P J McKenna; W A Muller; M M Zukowski; D P Andrew Journal: Eur J Immunol Date: 1998-06 Impact factor: 5.532
Authors: Nabeel A Arain; Luisangel Rondon; Deborah C Hogg; Jeffrey A Cadeddu; Richard Bergs; Raul Fernandez; Daniel J Scott Journal: Surg Endosc Date: 2012-05-31 Impact factor: 4.584
Authors: E Lezoche; M Guerrieri; A M Paganini; G D'Ambrosio; M Baldarelli; G Lezoche; F Feliciotti; A De Sanctis Journal: Surg Endosc Date: 2005-05-04 Impact factor: 4.584