Literature DB >> 12616714

TUNEL and immunofluorescence double-labeling assay for apoptotic cells with specific antigen(s).

Stephanie M Oberhaus1.   

Abstract

A double-labeling assay for detecting apoptotic cells, using the terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, and antigens of interest, using immunofluorescence, is described. The assay has been used successfully on fixed, cultured cells and formalin-fixed paraffin-embedded tissue sections. This in situ detection system can be used to correlate apoptosis with specific antigen expression to identify factors involved in the induction and execution of the apoptotic pathway of cell death in a wide variety of experimental systems.

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Year:  2003        PMID： 12616714     DOI： 10.1385/1-59259-356-9:85

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

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3.  β-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells.

Authors:  Zongze Wang; Ying Li; Fengxin Zhou; Zhe Piao; Jian Hao
Journal:  Oncol Lett       Date:  2018-06-06       Impact factor: 2.967

4.  Matrix modulation of compensatory lung regrowth and progenitor cell proliferation in mice.

Authors:  A M Hoffman; A Shifren; M R Mazan; A M Gruntman; K M Lascola; R D Nolen-Walston; C F Kim; L Tsai; R A Pierce; R P Mecham; E P Ingenito
Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2009-11-13       Impact factor: 5.464
  4 in total

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