Yutaka Shimomura1, Noriaki Aoki, Kaoru Ito, Masaaki Ito. 1. Department of Dermatology, Niigata University School of Medicine, Asahimachi-dori, Niigata 951-8510, Japan. yshimo@med.niigata-u.ac.jp
Abstract
BACKGROUND: Sh3yl1, which contains one Src homology (SH) 3 domain, has been previously identified from mouse skin and considered to play an important role in hair follicle formation by interacting with other proteins. OBJECTIVE: We performed this study to identify proteins capable of associating with Sh3yl1. METHODS: We screened a mouse skin cDNA library using the SH3 domain of Sh3yl1 as bait in the yeast two-hybrid system. RESULTS: We identified a 420-amino acid-long protein containing a proline-rich stretch and five carboxyl-terminal SH3 domains, which we have termed Sh3d19. We confirmed the interactions between Sh3yl1 and Sh3d19 by in vitro binding assays. Northern blot analysis showed that Sh3d19 transcripts in mouse skin were expressed in accordance with the hair cycle. Furthermore, RNA in situ hybridization studies demonstrated that its transcripts were detected predominantly in the medulla cells at the level corresponding to the keratogenous zone of the hair follicles during the mid and late anagen phases. CONCLUSION: Sh3d19 is a novel adaptor protein that may be involved in the development of medulla cells during the anagen phase.
BACKGROUND:Sh3yl1, which contains one Src homology (SH) 3 domain, has been previously identified from mouse skin and considered to play an important role in hair follicle formation by interacting with other proteins. OBJECTIVE: We performed this study to identify proteins capable of associating with Sh3yl1. METHODS: We screened a mouse skin cDNA library using the SH3 domain of Sh3yl1 as bait in the yeast two-hybrid system. RESULTS: We identified a 420-amino acid-long protein containing a proline-rich stretch and five carboxyl-terminal SH3 domains, which we have termed Sh3d19. We confirmed the interactions between Sh3yl1 and Sh3d19 by in vitro binding assays. Northern blot analysis showed that Sh3d19 transcripts in mouse skin were expressed in accordance with the hair cycle. Furthermore, RNA in situ hybridization studies demonstrated that its transcripts were detected predominantly in the medulla cells at the level corresponding to the keratogenous zone of the hair follicles during the mid and late anagen phases. CONCLUSION:Sh3d19 is a novel adaptor protein that may be involved in the development of medulla cells during the anagen phase.
Authors: Yutaka Shimomura; Dritan Agalliu; Alin Vonica; Victor Luria; Muhammad Wajid; Alessandra Baumer; Serena Belli; Lynn Petukhova; Albert Schinzel; Ali H Brivanlou; Ben A Barres; Angela M Christiano Journal: Nature Date: 2010-04-15 Impact factor: 49.962
Authors: Alicia M Blessing; Sathya Ganesan; Kimal Rajapakshe; Ying Ying Sung; Lakshmi Reddy Bollu; Yan Shi; Edwin Cheung; Cristian Coarfa; Jeffrey T Chang; Donald P McDonnell; Daniel E Frigo Journal: Mol Endocrinol Date: 2015-08-25