BACKGROUND: The optimal technique for producing linear radiofrequency thermal lesions in myocardial tissue is unclear. We compared epicardial ablation on the beating heart with endocardial ablation after cardioplegia. METHODS: Radiofrequency lesions were produced using a multielectrode malleable handheld probe in ovine myocardium with three wall thicknesses. Detailed analysis of lesion dimensions was used to assess the effects of site of ablation, muscle thickness, and duration of ablation. RESULTS: After epicardial atrial ablation, myocardial lesions were detected in all sections without macroscopically visible epicardial fat (n = 10), but only 43% (6/14) of sections with epicardial fat. Three of 24 atrial epicardial sections (13%) and 92% (23/25) of endocardial atrial lesion sections were clearly transmural. In thicker tissues lesion depth was independent of endocardial (right ventricle: 3.9 +/- 1.1 mm, left ventricle: 3.8 +/- 0.7 mm) or epicardial (right ventricle: 3.4 +/- 0.6 mm, left ventricle: 4.3 +/- 0.9 mm) ablation site. Epicardial lesions are less deep in thinner areas of myocardium (p = 0.003). Lesions were all wider than they were deep. There was no significant increase in lesion depth with the increase in ablation duration from 1 to 2 minutes. CONCLUSIONS: Lesions were unlikely to be transmural with either technique when the wall thickness was greater than about 4 mm. Epicardial fat has an important negative effect on epicardial lesion formation. Where epicardial fat is absent epicardially produced lesions penetrate less deeply when the wall thickness is small, possibly due to endocardial cooling by circulating blood. Prolongation of the duration of ablation from 1 to 2 minutes does not significantly increase lesion depth.
BACKGROUND: The optimal technique for producing linear radiofrequency thermal lesions in myocardial tissue is unclear. We compared epicardial ablation on the beating heart with endocardial ablation after cardioplegia. METHODS: Radiofrequency lesions were produced using a multielectrode malleable handheld probe in ovine myocardium with three wall thicknesses. Detailed analysis of lesion dimensions was used to assess the effects of site of ablation, muscle thickness, and duration of ablation. RESULTS: After epicardial atrial ablation, myocardial lesions were detected in all sections without macroscopically visible epicardial fat (n = 10), but only 43% (6/14) of sections with epicardial fat. Three of 24 atrial epicardial sections (13%) and 92% (23/25) of endocardial atrial lesion sections were clearly transmural. In thicker tissues lesion depth was independent of endocardial (right ventricle: 3.9 +/- 1.1 mm, left ventricle: 3.8 +/- 0.7 mm) or epicardial (right ventricle: 3.4 +/- 0.6 mm, left ventricle: 4.3 +/- 0.9 mm) ablation site. Epicardial lesions are less deep in thinner areas of myocardium (p = 0.003). Lesions were all wider than they were deep. There was no significant increase in lesion depth with the increase in ablation duration from 1 to 2 minutes. CONCLUSIONS: Lesions were unlikely to be transmural with either technique when the wall thickness was greater than about 4 mm. Epicardial fat has an important negative effect on epicardial lesion formation. Where epicardial fat is absent epicardially produced lesions penetrate less deeply when the wall thickness is small, possibly due to endocardial cooling by circulating blood. Prolongation of the duration of ablation from 1 to 2 minutes does not significantly increase lesion depth.
Authors: Hugh Calkins; Gerhard Hindricks; Riccardo Cappato; Young-Hoon Kim; Eduardo B Saad; Luis Aguinaga; Joseph G Akar; Vinay Badhwar; Josep Brugada; John Camm; Peng-Sheng Chen; Shih-Ann Chen; Mina K Chung; Jens Cosedis Nielsen; Anne B Curtis; D Wyn Davies; John D Day; André d'Avila; N M S Natasja de Groot; Luigi Di Biase; Mattias Duytschaever; James R Edgerton; Kenneth A Ellenbogen; Patrick T Ellinor; Sabine Ernst; Guilherme Fenelon; Edward P Gerstenfeld; David E Haines; Michel Haissaguerre; Robert H Helm; Elaine Hylek; Warren M Jackman; Jose Jalife; Jonathan M Kalman; Josef Kautzner; Hans Kottkamp; Karl Heinz Kuck; Koichiro Kumagai; Richard Lee; Thorsten Lewalter; Bruce D Lindsay; Laurent Macle; Moussa Mansour; Francis E Marchlinski; Gregory F Michaud; Hiroshi Nakagawa; Andrea Natale; Stanley Nattel; Ken Okumura; Douglas Packer; Evgeny Pokushalov; Matthew R Reynolds; Prashanthan Sanders; Mauricio Scanavacca; Richard Schilling; Claudio Tondo; Hsuan-Ming Tsao; Atul Verma; David J Wilber; Teiichi Yamane Journal: Heart Rhythm Date: 2017-05-12 Impact factor: 6.343