Literature DB >> 12605863

A quantitative diffraction-based sandwich immunoassay.

Jane B Goh1, Pui L Tam, Richard W Loo, M Cynthia Goh.   

Abstract

It is shown that diffraction-based sensing can be enhanced for diagnostic purposes through the use of a secondary label. The limit of detection for anti-rabbit IgG was reduced more than 40-fold by using a gold-conjugated secondary antibody. The response to secondary antibody binding was linear for concentrations from 25 to 500 ng/ml of anti-rabbit IgG, suggesting that quantitative determinations can be readily done. Moreover, the binding of the secondary antibody was observed as soon as 1 min after its introduction to the surface-bound primary complex.

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Year:  2003        PMID： 12605863     DOI： 10.1016/s0003-2697(02)00634-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Electrophoretic build-up of multi nanoparticle array for a highly sensitive immunoassay.

Authors:  Jin-Hee Han; Hee-Joo Kim; L Sudheendra; Elizabeth A Hass; Shirley J Gee; Bruce D Hammock; Ian M Kennedy
Journal:  Biosens Bioelectron       Date:  2012-09-06       Impact factor: 10.618

2.  Molecular layer detection on a diffractive optical balance.

Authors:  Xuefeng Wang; Ming Zhao; David D Nolte
Journal:  Opt Lett       Date:  2012-10-01       Impact factor: 3.776

3.  Nanoparticle diffraction gratings for DNA detection on photopatterned glass substrates.

Authors:  Iuliana E Sendroiu; Robert M Corn
Journal:  Biointerphases       Date:  2008-09       Impact factor: 2.456

4.  A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium.

Authors:  Palaniappan Ramasamy; Gajalakshmi Dakshinamoorthy; Shanmugam Jayashree; Dhamodharan Prabhu; Sundararaj Rajamanikandan; Palaniyandi Velusamy; Govindan Dayanithi; Robert E B Hanna
Journal:  Biosensors (Basel)       Date:  2022-06-04

5.  Diffractometric detection of proteins using microbead-based rolling circle amplification.

Authors:  Joonhyung Lee; Kutay Icoz; Ana Roberts; Andrew D Ellington; Cagri A Savran
Journal:  Anal Chem       Date:  2010-01-01       Impact factor: 6.986

6.  Ultrasensitive on-chip immunoassays with a nanoparticle-assembled photonic crystal.

Authors:  Jin-Hee Han; L Sudheendra; Hee-Joo Kim; Shirley J Gee; Bruce D Hammock; Ian M Kennedy
Journal:  ACS Nano       Date:  2012-09-14       Impact factor: 15.881

7.  Characterization of the interaction of staphylococcal enterotoxin B with CD1d expressed in human renal proximal tubule epithelial cells.

Authors:  Rasha Hammamieh; Nabarun Chakraborty; Yixin Lin; Jeffrey W Shupp; Stacy-Ann Miller; Sam Morris; Marti Jett
Journal:  BMC Microbiol       Date:  2015-02-04       Impact factor: 3.605

8.  Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology.

Authors:  Brian J Pak; Fabio Vasquez-Camargo; Evgeniya Kalinichenko; Peter L Chiodini; Thomas B Nutman; Herbert B Tanowitz; Isabel McAuliffe; Patricia Wilkins; Paul T Smith; Brian J Ward; Michael D Libman; Momar Ndao
Journal:  PLoS Negl Trop Dis       Date:  2014-08-07
  8 in total

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