Effect of some inhibitors on aflatoxin-production in a synthetic medium and on the incorporation of acetate-1- 14C into aflatoxins by resting mycelia of Aspergillus parasiticus.

The effect of a number of metabolic inhibitors on the incorporation of acetate-1-14C into aflatoxins was investigated, using resting mycelia of Aspergillus parasiticus suspended in phosphate buffer. Malonate, iodoacetate, sodium arsenite, 2:4 dinitrophenol, sodium fluoride and p-aminosalicylate stimulated the incorporation at low concentrations and inhibited the same at high concentrations. p-Nitrobenzoic acid was inhibitory at all the concentrations tried. Fluoride, arsenite, arsenate and iodoacetate inhibited both growth and aflatoxin production when added directly to the growth medium. In general, there was a greater inhibition in growth medium than with the suspended mycelia.