Matthew I Bonaparte1, Edward Barker. 1. Department of Microbiology and Immunology, University of New York, Upstate Medical University, Syracuse, New York 13210, USA.
Abstract
OBJECTIVE: Determine whether natural killer (NK) cells are capable of killing HIV-infected autologous primary T-cell blasts. DESIGN: The ability of NK cells to kill HIV-infected primary T-cell blasts, whose cell surface major histocompatibility complex (MHC) class I molecules was decreased, was evaluated in a lytic assay. METHODS: Phytohemagglutinin-treated CD4+ T cells were infected with HIV-1. Infected cells were separated from uninfected cells by removal of CD4+ T cells. The NK cells were isolated from peripheral blood mononuclear cells (PBMC) of the same donor as the CD4+ T cells by immunomagnetic bead separation. The NK cells isolated from PBMC were then used as effector cells and the HIV-infected T-cell blasts were used as target cells in a lytic assay. RESULTS: It was demonstrated that HIV infection of primary CD4+ T cells results in a 61-68% reduction in surface expression of MHC class I molecules. Despite the decreased MHC class I expression the NK cells were incapable of lysing autologous HIV-infected T-cell blasts, yet were effective in the lysis of the NK cell sensitive cell line, K562. The inability of NK cells to lyse HIV-infected T-cell blasts is not dependent on the strain of HIV used to infected the CD4+ T cell CONCLUSION: These studies indicate that despite drastic decreases in MHC class I molecule expression, HIV-infected T-cell blasts can evade destruction by autologous NK cells.
OBJECTIVE: Determine whether natural killer (NK) cells are capable of killing HIV-infected autologous primary T-cell blasts. DESIGN: The ability of NK cells to kill HIV-infected primary T-cell blasts, whose cell surface major histocompatibility complex (MHC) class I molecules was decreased, was evaluated in a lytic assay. METHODS: Phytohemagglutinin-treated CD4+ T cells were infected with HIV-1. Infected cells were separated from uninfected cells by removal of CD4+ T cells. The NK cells were isolated from peripheral blood mononuclear cells (PBMC) of the same donor as the CD4+ T cells by immunomagnetic bead separation. The NK cells isolated from PBMC were then used as effector cells and the HIV-infected T-cell blasts were used as target cells in a lytic assay. RESULTS: It was demonstrated that HIV infection of primary CD4+ T cells results in a 61-68% reduction in surface expression of MHC class I molecules. Despite the decreased MHC class I expression the NK cells were incapable of lysing autologous HIV-infected T-cell blasts, yet were effective in the lysis of the NK cell sensitive cell line, K562. The inability of NK cells to lyse HIV-infected T-cell blasts is not dependent on the strain of HIV used to infected the CD4+ T cell CONCLUSION: These studies indicate that despite drastic decreases in MHC class I molecule expression, HIV-infected T-cell blasts can evade destruction by autologous NK cells.
Authors: Ankur H Shah; Bharatwaj Sowrirajan; Zachary B Davis; Jeffrey P Ward; Edward M Campbell; Vicente Planelles; Edward Barker Journal: Cell Host Microbe Date: 2010-11-18 Impact factor: 21.023
Authors: Julian J Lum; David J Schnepple; Zilin Nie; Jaime Sanchez-Dardon; Georgina L Mbisa; Jennifer Mihowich; Nanci Hawley; Shanil Narayan; John E Kim; David H Lynch; Andrew D Badley Journal: J Virol Date: 2004-06 Impact factor: 5.103