Literature DB >> 12597884

Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli.

Yan-Feng Liu1, Run-Lin Ma, Si-Ling Wang, Zi-Yuan Duan, Jin-Hai Zhang, Li-Jun Wu, Chun-Fu Wu.   

Abstract

The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice. Copyright 2002 Elsevier Science (USA)

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Year:  2003        PMID： 12597884     DOI： 10.1016/s1046-5928(02)00609-5

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  29 in total

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Review 4.  Helminthes and insects: maladies or therapies.

Authors:  Nora L El-Tantawy
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5.  The expression of analgesic-antitumor peptide (AGAP) from Chinese Buthus martensii Karsch in transgenic tobacco and tomato.

Authors:  Linlin Lai; Tingting Huang; Yi Wang; Yanfeng Liu; Jinghai Zhang; Yongbo Song
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7.  The role of glycine residues at the C-terminal peptide segment in antinociceptive activity: a molecular dynamics simulation.

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8.  Homology modeling and molecular dynamics simulation of odonthubuthus doriae (Od1) scorpion toxin in comparison to the BmK M1.

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Journal:  Bioinformation       Date:  2012-05-31

9.  Scorpion peptides: potential use for new drug development.

Authors:  Bennasr Hmed; Hammami Turky Serria; Zeghal Khaled Mounir
Journal:  J Toxicol       Date:  2013-06-15

10.  BmK-YA, an enkephalin-like peptide in scorpion venom.

Authors:  Yan Zhang; Junyan Xu; Zhiwei Wang; Xiuli Zhang; Xinmiao Liang; Olivier Civelli
Journal:  PLoS One       Date:  2012-07-09       Impact factor: 3.240
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