Literature DB >> 1259718

Investigation of ribonuclease-catalysed kinetics by a micro-calorimetric method.

M Tribout, S Paredes, J Léonis.   

Abstract

A rapid micro-calorimetric method for the simultaneous determination of the Michaelis-Menten parameters and the enthalpy of enzymic reactions is developed. The hydrolysis of 2': 3'-cyclic CMP by ribonuclease A is studied to test the proposed method; values obtained are in good agreement with already published data. Enzymic hydrolysis of yeast RNA, unlike that of cyclic phosphates, is shown to be endothermic. This result is explained by the two-step mechanism of this reaction.

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Year:  1976        PMID： 1259718      PMCID： PMC1172544          DOI： 10.1042/bj1530089

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  Mechanisms related to enzyme catalysis.

Authors:  F H WESTHEIMER
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1962

2.  Mechanism and binding sites in the ribonuclease reaction. II. Kinetic studies on the first step of the reaction.

Authors:  H WITZEL; E A BARNARD
Journal:  Biochem Biophys Res Commun       Date:  1962-05-04       Impact factor: 3.575

3.  Mechanism and binding sites in the ribonuclease reaction. I. Kinetic studies on the second step of the reaction.

Authors:  H WITZEL; E A BARNARD
Journal:  Biochem Biophys Res Commun       Date:  1962-05-04       Impact factor: 3.575

4.  [Some factors which affect the enzymatic digestion of ribonucleic acid].

Authors:  G KALNITSKY; J P HUMMEL; C DIERKS
Journal:  J Biol Chem       Date:  1959-06       Impact factor: 5.157

5.  Spectrophotometric assay of bovine pancreatic ribonuclease by the use of cytidine 2':3'-phosphate.

Authors:  E M CROOK; A P MATHIAS; B R RABIN
Journal:  Biochem J       Date:  1960-02       Impact factor: 3.857

6.  The enthalpy of hydrolysis of various 3',5'-and 2',3'-cyclic nucleotides.

Authors:  S A Rudolph; E M Johnson; P Greengard
Journal:  J Biol Chem       Date:  1971-03-10       Impact factor: 5.157

7.  Flow microcalorimetry as an analytical tool in biochemistry and related areas.

Authors:  P Monk; I Wadsö
Journal:  Acta Chem Scand       Date:  1969

8.  Enthalpy of hydrolysis of the 3' bond of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate.

Authors:  P Greengard; S A Rudolph; J M Sturtevant
Journal:  J Biol Chem       Date:  1969-09-10       Impact factor: 5.157
  8 in total
  1 in total

1.  Isothermal titration calorimetric study of RNase-A kinetics (cCMP --> 3'-CMP) involving end-product inhibition.

Authors:  Shawn D Spencer; Robert B Raffa
Journal:  Pharm Res       Date:  2004-09       Impact factor: 4.200
  1 in total

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