C Vasu1, J Vadivelu, S D Puthucheary. 1. Dept of Microbiology and Immunology, E-709, University of Illinois at Chicago, 835 S Wolcott, Chicago, IL-60612, USA. chenta@uic.edu
Abstract
BACKGROUND: This study was undertaken to identify and quantify the class and subclass antibody responses to the culture filtrate antigen (CFA) of Burkholderia pseudomallei in melioidosis patients under long-term maintenance or eradication therapy. MATERIALS AND METHODS: Sequential sera samples from seven melioidosis patients collected between January 1992 and April 1998 were analyzed for immunoglobulin (Ig) types and IgG isotypes by ELISA using B. pseudomallei CFA. RESULTS: Melioidosis patients generated a strong IgG, IgA and IgM response to the CFA of B. pseudomallei throughout the infection and IgG1 and IgG2 were the predominant IgG istotypes produced. Although high levels of these antibodies were detected in all the seven patients, the IgG, IgG1 and IgG2 antibodies showed a consistent response and good correlation with the clinical history in all cases. CONCLUSION: This study suggests that monitoring IgG antibody or IgG1 or IgG2 isotype antibody levels to CFA in patients under maintenance or eradication antibiotic therapy may be useful as a tool to detect the status of infection and as a guideline to determine the duration of maintenance antimicrobial therapy.
BACKGROUND: This study was undertaken to identify and quantify the class and subclass antibody responses to the culture filtrate antigen (CFA) of Burkholderia pseudomallei in melioidosispatients under long-term maintenance or eradication therapy. MATERIALS AND METHODS: Sequential sera samples from seven melioidosispatients collected between January 1992 and April 1998 were analyzed for immunoglobulin (Ig) types and IgG isotypes by ELISA using B. pseudomallei CFA. RESULTS:Melioidosispatients generated a strong IgG, IgA and IgM response to the CFA of B. pseudomallei throughout the infection and IgG1 and IgG2 were the predominant IgG istotypes produced. Although high levels of these antibodies were detected in all the seven patients, the IgG, IgG1 and IgG2 antibodies showed a consistent response and good correlation with the clinical history in all cases. CONCLUSION: This study suggests that monitoring IgG antibody or IgG1 or IgG2 isotype antibody levels to CFA in patients under maintenance or eradication antibiotic therapy may be useful as a tool to detect the status of infection and as a guideline to determine the duration of maintenance antimicrobial therapy.
Authors: Vanaporn Wuthiekanun; Premjit Amornchai; Wirongrong Chierakul; Allen C Cheng; Nicholas J White; Sharon J Peacock; Nicholas P J Day Journal: J Clin Microbiol Date: 2004-08 Impact factor: 5.948
Authors: Natasha Patel; Laura Conejero; Melanie De Reynal; Anna Easton; Gregory J Bancroft; Richard W Titball Journal: Front Microbiol Date: 2011-09-27 Impact factor: 5.640